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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80811 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MICA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MICA Polyclonal Antibody (Product # PA5-80811) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: Daudi Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 55 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human MICA in human breast-carcinoma with MICA Polyclonal Antibody (Product # PA5-80811, 1:1,000, formalin-fixed paraffin embedded sections). Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MICA Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 2 µL with MICA Polyclonal Antibody (Product # PA5-80811) and 15 µL of 50 % Protein G agarose. Primary antibody: MICA Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.
