Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-588 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-588, RRID:AB_2150118
- Product name
- Rabbit Polyclonal Nanog Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references YTHDF2 promotes the liver cancer stem cell phenotype and cancer metastasis by regulating OCT4 expression via m6A RNA methylation.
Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging.
Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment.
Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells.
Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m⁶A-demethylation of NANOG mRNA.
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
Low-dose 6-bromoindirubin-3'-oxime induces partial dedifferentiation of endothelial cells to promote increased neovascularization.
Reprogramming to pluripotency can conceal somatic cell chromosomal instability.
SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2.
Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion.
Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells.
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Reprogramming of murine and human somatic cells using a single polycistronic vector.
Zhang C, Huang S, Zhuang H, Ruan S, Zhou Z, Huang K, Ji F, Ma Z, Hou B, He X
Oncogene 2020 Jun;39(23):4507-4518
Oncogene 2020 Jun;39(23):4507-4518
Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging.
Daum R, Brauchle EM, Berrio DAC, Jurkowski TP, Schenke-Layland K
Scientific reports 2019 May 7;9(1):7014
Scientific reports 2019 May 7;9(1):7014
Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment.
Lu H, Chen I, Shimoda LA, Park Y, Zhang C, Tran L, Zhang H, Semenza GL
Cell reports 2017 Feb 21;18(8):1946-1957
Cell reports 2017 Feb 21;18(8):1946-1957
Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells.
Zhang C, Zhi WI, Lu H, Samanta D, Chen I, Gabrielson E, Semenza GL
Oncotarget 2016 Oct 4;7(40):64527-64542
Oncotarget 2016 Oct 4;7(40):64527-64542
Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m⁶A-demethylation of NANOG mRNA.
Zhang C, Samanta D, Lu H, Bullen JW, Zhang H, Chen I, He X, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 5;113(14):E2047-56
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 5;113(14):E2047-56
Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.
Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9
Low-dose 6-bromoindirubin-3'-oxime induces partial dedifferentiation of endothelial cells to promote increased neovascularization.
Kohler EE, Baruah J, Urao N, Ushio-Fukai M, Fukai T, Chatterjee I, Wary KK
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1538-52
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1538-52
Reprogramming to pluripotency can conceal somatic cell chromosomal instability.
Hamada M, Malureanu LA, Wijshake T, Zhou W, van Deursen JM
PLoS genetics 2012;8(8):e1002913
PLoS genetics 2012;8(8):e1002913
SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2.
Wu Y, Guo Z, Wu H, Wang X, Yang L, Shi X, Du J, Tang B, Li W, Yang L, Zhang Y
PloS one 2012;7(6):e39606
PloS one 2012;7(6):e39606
Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion.
Ganuza M, Sáiz-Ladera C, Cañamero M, Gómez G, Schneider R, Blasco MA, Pisano D, Paramio JM, Santamaría D, Barbacid M
The EMBO journal 2012 May 30;31(11):2498-510
The EMBO journal 2012 May 30;31(11):2498-510
Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells.
Morgado-Palacin L, Llanos S, Serrano M
Cell cycle (Georgetown, Tex.) 2012 Feb 1;11(3):503-10
Cell cycle (Georgetown, Tex.) 2012 Feb 1;11(3):503-10
NANOG induction of fetal liver kinase-1 (FLK1) transcription regulates endothelial cell proliferation and angiogenesis.
Kohler EE, Cowan CE, Chatterjee I, Malik AB, Wary KK
Blood 2011 Feb 3;117(5):1761-9
Blood 2011 Feb 3;117(5):1761-9
Reprogramming of murine and human somatic cells using a single polycistronic vector.
Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):157-62
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 6;106(1):157-62
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-588] - The expression of pluripotency factor Nanog in human head and neck cancer cell lines cell lines FaDu and HN5. WB from a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody [NB100-588] - Detection of mouse Nanog by western blot. Samples: Whole cell lysate (50 ug) from F9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nanog antibody NB100-588 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2 cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.