EB07693

antibody from Everest Biotech

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

EB07693

Provider

Everest Biotech

Proper citation

Everest Biotech Cat#EB07693, RRID:AB_2150392

Product name

Goat anti-NANOG (C Terminus) Antibody

Antibody type

Polyclonal

Description

Goat anti-NANOG (C Terminus) Antibody

Reactivity

Human

Host

Goat

Antigen sequence

C-DLFLNYSMNMQPED


Epitope

C Terminus

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07693 (0.05µg/ml) staining of HeLa (nuclear) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB07693 (2µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.