Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX100863 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100863, RRID:AB_10615506
- Product name
- Nanog antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Generation of a human Charcot-Marie-Tooth disease type 1B (CMT1B) iPSC line, ZJUCHi001-A, with a mutation of c.292C>T in MPZ.
Spheroids from adipose-derived stem cells exhibit an miRNA profile of highly undifferentiated cells.
Embryos and embryonic stem cells from the white rhinoceros.
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Enforcement of developmental lineage specificity by transcription factor Oct1.
Pro-differentiating effects of a synthetic flavagline on human teratocarcinomal cancer stem-like cells.
Suberoylanilide hydroxamic acid represses glioma stem-like cells.
Systematic optimization of human pluripotent stem cells media using Design of Experiments.
Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells.
Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.
Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway.
Arsenic inhibits hedgehog signaling during P19 cell differentiation.
Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
Directed differentiation of human pluripotent cells to neural crest stem cells.
Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the β-catenin signaling pathway.
Xu J, Wang Y, He J, Xia W, Zou Y, Ruan W, Lou Q, Li Y, Li H, Chen W
Stem cell research 2019 Mar;35:101407
Stem cell research 2019 Mar;35:101407
Spheroids from adipose-derived stem cells exhibit an miRNA profile of highly undifferentiated cells.
Di Stefano AB, Grisafi F, Castiglia M, Perez A, Montesano L, Gulino A, Toia F, Fanale D, Russo A, Moschella F, Leto Barone AA, Cordova A
Journal of cellular physiology 2018 Nov;233(11):8778-8789
Journal of cellular physiology 2018 Nov;233(11):8778-8789
Embryos and embryonic stem cells from the white rhinoceros.
Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C
Nature communications 2018 Jul 4;9(1):2589
Nature communications 2018 Jul 4;9(1):2589
A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Ni YL, Hsieh CH, Kim SH, Wang JP, Su CL, Yao CF, Fang K
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Apoptosis : an international journal on programmed cell death 2017 Oct;22(10):1235-1245
Enforcement of developmental lineage specificity by transcription factor Oct1.
Shen Z, Kang J, Shakya A, Tabaka M, Jarboe EA, Regev A, Tantin D
eLife 2017 May 24;6
eLife 2017 May 24;6
Pro-differentiating effects of a synthetic flavagline on human teratocarcinomal cancer stem-like cells.
Emhemmed F, Ali Azouaou S, Zhao Q, Appert-Collin A, Bennasroune A, Schini-Kerth VB, Muller CD, Désaubry L, Fuhrmann G
Cell biology and toxicology 2017 Jun;33(3):295-306
Cell biology and toxicology 2017 Jun;33(3):295-306
Suberoylanilide hydroxamic acid represses glioma stem-like cells.
Hsu CC, Chang WC, Hsu TI, Liu JJ, Yeh SH, Wang JY, Liou JP, Ko CY, Chang KY, Chuang JY
Journal of biomedical science 2016 Nov 18;23(1):81
Journal of biomedical science 2016 Nov 18;23(1):81
Systematic optimization of human pluripotent stem cells media using Design of Experiments.
Marinho PA, Chailangkarn T, Muotri AR
Scientific reports 2015 May 5;5:9834
Scientific reports 2015 May 5;5:9834
Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells.
Ju SY, Huang CY, Huang WC, Su Y
Cell death & disease 2015 Jul 2;6(7):e1801
Cell death & disease 2015 Jul 2;6(7):e1801
Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.
Mineda K, Feng J, Ishimine H, Takada H, Doi K, Kuno S, Kinoshita K, Kanayama K, Kato H, Mashiko T, Hashimoto I, Nakanishi H, Kurisaki A, Yoshimura K
Stem cells translational medicine 2015 Dec;4(12):1511-22
Stem cells translational medicine 2015 Dec;4(12):1511-22
Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway.
Emhemmed F, Ali Azouaou S, Thuaud F, Schini-Kerth V, Désaubry L, Muller CD, Fuhrmann G
Biochemical pharmacology 2014 May 15;89(2):185-96
Biochemical pharmacology 2014 May 15;89(2):185-96
Arsenic inhibits hedgehog signaling during P19 cell differentiation.
Liu JT, Bain LJ
Toxicology and applied pharmacology 2014 Dec 15;281(3):243-53
Toxicology and applied pharmacology 2014 Dec 15;281(3):243-53
Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells.
Singh AM, Chappell J, Trost R, Lin L, Wang T, Tang J, Matlock BK, Weller KP, Wu H, Zhao S, Jin P, Dalton S
Stem cell reports 2013;1(6):532-44
Stem cell reports 2013;1(6):532-44
Directed differentiation of human pluripotent cells to neural crest stem cells.
Menendez L, Kulik MJ, Page AT, Park SS, Lauderdale JD, Cunningham ML, Dalton S
Nature protocols 2013 Jan;8(1):203-12
Nature protocols 2013 Jan;8(1):203-12
Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the β-catenin signaling pathway.
Hong GM, Bain LJ
Toxicological sciences : an official journal of the Society of Toxicology 2012 Sep;129(1):146-56
Toxicological sciences : an official journal of the Society of Toxicology 2012 Sep;129(1):146-56
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug ) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa nucleus 12% SDS PAGE GTX100863 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 ?g/mL and GTX89079 for detection at a concentration of 5 ?g/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG(GTX100863) antibody at 1:400 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.Green: NANOG protein stained by NANOG antibody [N3C3] (GTX100863) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 6 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 £gg of NANOG antibody [N3C3] (GTX100863).Western blot analysis was performed using NANOG antibody [N3C3] (GTX100863).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.