Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA302153 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against NANOG (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against NANOG (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NANOG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
Submitted references CHI3L1 results in poor outcome of ovarian cancer by promoting properties of stem-like cells.
Lin HW, Chiang YC, Sun NY, Chen YL, Chang CF, Tai YJ, Chen CA, Cheng WF
Endocrine-related cancer 2019 Jan 1;26(1):73-88
Endocrine-related cancer 2019 Jan 1;26(1):73-88
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with NANOG Antibody (N-term)(Cat#TA302153).Hela cells were incubated with NANOG primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NANOG immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC