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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- PAB7232 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7232, RRID:AB_1577572
- Product name
- NANOG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NANOG.
- Antigen sequence
C-DLFLNYSMNMQPED- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer.
Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H
Genes to cells : devoted to molecular & cellular mechanisms 2006 Sep;11(9):1115-23
Genes to cells : devoted to molecular & cellular mechanisms 2006 Sep;11(9):1115-23
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NANOG polyclonal antibody (Cat # PAB7232) (0.05 ug/mL) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NANOG polyclonal antibody (Cat # PAB7232) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
- Validation comment
- Sandwich ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NANOG polyclonal antibody (Cat # PAB7232) (2 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
