Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-04320 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-04320, RRID:AB_1521950
- Product name
- Mouse Monoclonal Nanog Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nodal signaling activates the Smad2/3 pathway to regulate stem cell-like properties in breast cancer cells.
Bazedoxifene enhances the anti-tumor effects of cisplatin and radiation treatment by blocking IL-6 signaling in head and neck cancer.
Characterization of Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma.
Embryonic Stem Cell-like Population in Dupuytren's Disease.
Suberoylanilide hydroxamic acid (SAHA) reverses chemoresistance in head and neck cancer cells by targeting cancer stem cells via the downregulation of nanog.
Mouse and human induced pluripotent stem cells as a source for multipotent Isl1+ cardiovascular progenitors.
Gong W, Sun B, Sun H, Zhao X, Zhang D, Liu T, Zhao N, Gu Q, Dong X, Liu F
American journal of cancer research 2017;7(3):503-517
American journal of cancer research 2017;7(3):503-517
Bazedoxifene enhances the anti-tumor effects of cisplatin and radiation treatment by blocking IL-6 signaling in head and neck cancer.
Yadav A, Kumar B, Teknos TN, Kumar P
Oncotarget 2017 Sep 15;8(40):66912-66924
Oncotarget 2017 Sep 15;8(40):66912-66924
Characterization of Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma.
Yu HH, Featherston T, Tan ST, Chibnall AM, Brasch HD, Davis PF, Itinteang T
Frontiers in surgery 2016;3:46
Frontiers in surgery 2016;3:46
Embryonic Stem Cell-like Population in Dupuytren's Disease.
Koh SP, On N, Brasch HD, Chibnall AM, Armstrong JR, Davis PF, Tan ST, Itinteang T
Plastic and reconstructive surgery. Global open 2016 Nov;4(11):e1064
Plastic and reconstructive surgery. Global open 2016 Nov;4(11):e1064
Suberoylanilide hydroxamic acid (SAHA) reverses chemoresistance in head and neck cancer cells by targeting cancer stem cells via the downregulation of nanog.
Kumar B, Yadav A, Lang JC, Teknos TN, Kumar P
Genes & cancer 2015 Mar;6(3-4):169-81
Genes & cancer 2015 Mar;6(3-4):169-81
Mouse and human induced pluripotent stem cells as a source for multipotent Isl1+ cardiovascular progenitors.
Moretti A, Bellin M, Jung CB, Thies TM, Takashima Y, Bernshausen A, Schiemann M, Fischer S, Moosmang S, Smith AG, Lam JT, Laugwitz KL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Mar;24(3):700-11
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Mar;24(3):700-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.