Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [4]
- Flow cytometry [1]
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- Product number
- GTX627421 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX627421, RRID:AB_11169335
- Product name
- Nanog antibody [GT3312]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references The antipsychotic chlorpromazine suppresses YAP signaling, stemness properties, and drug resistance in breast cancer cells.
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.
Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Stress granule-associated protein G3BP2 regulates breast tumor initiation.
The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.
Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.
Yang CE, Lee WY, Cheng HW, Chung CH, Mi FL, Lin CW
Chemico-biological interactions 2019 Apr 1;302:28-35
Chemico-biological interactions 2019 Apr 1;302:28-35
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Fan JR, Lee HT, Lee W, Lin CH, Hsu CY, Hsieh CH, Shyu WC
Cell death & disease 2018 May 1;9(5):502
Cell death & disease 2018 May 1;9(5):502
Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
Lin YH, Wu MH, Huang YH, Yeh CT, Chi HC, Tsai CY, Chuang WY, Yu CJ, Chung IH, Chen CY, Lin KH
Endocrine-related cancer 2018 Dec 1;25(12):967-979
Endocrine-related cancer 2018 Dec 1;25(12):967-979
MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.
Ko CY, Lin CH, Chuang JY, Chang WC, Hsu TI
Molecular neurobiology 2018 Apr;55(4):3211-3223
Molecular neurobiology 2018 Apr;55(4):3211-3223
Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
Chang KY, Huang CT, Hsu TI, Hsu CC, Liu JJ, Chuang CK, Hung JJ, Chang WC, Tsai KK, Chuang JY
Biochemical and biophysical research communications 2017 Nov 4;493(1):14-19
Biochemical and biophysical research communications 2017 Nov 4;493(1):14-19
Stress granule-associated protein G3BP2 regulates breast tumor initiation.
Gupta N, Badeaux M, Liu Y, Naxerova K, Sgroi D, Munn LL, Jain RK, Garkavtsev I
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 31;114(5):1033-1038
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 31;114(5):1033-1038
The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
Huang TT, Ping YH, Wang AM, Ke CC, Fang WL, Huang KH, Lee HC, Chi CW, Yeh TS
Oncotarget 2015 Jul 20;6(20):18012-26
Oncotarget 2015 Jul 20;6(20):18012-26
Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
Wang AM, Huang TT, Hsu KW, Huang KH, Fang WL, Yang MH, Lo SS, Chi CW, Lin JJ, Yeh TS
Oncotarget 2014 Jul 15;5(13):5002-16
Oncotarget 2014 Jul 15;5(13):5002-16
CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.
Huang AF, Chen MW, Huang SM, Kao CL, Lai HC, Chan JY
Molecular cancer 2013 Oct 5;12(1):115
Molecular cancer 2013 Oct 5;12(1):115
Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.
Liu KC, Yo YT, Huang RL, Wang YC, Liao YP, Huang TS, Chao TK, Lin CK, Weng SJ, Ma KH, Chang CC, Yu MH, Lai HC
Oncotarget 2013 Dec;4(12):2366-82
Oncotarget 2013 Dec;4(12):2366-82
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NANOG antibody [GT3312] validation by siRNA knock-down.Upperpanel: NANOG antibody [GT3312] GTX627421Lower panel: GAPDH antibody (GTX100118)A. 30 ?g Tera-2 whole cell lysate/extract B. 30 ?g whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells C. 30 ?g whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells D. 30 ?g whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells 10% SDS-PAGENANOG antibody [GT3312] (GTX627421) dilution: 1:1000GAPDH antibody (GTX100118) dilution: 1:10000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NANOG antibody [GT3312] detects NANOG protein by western blot analysis.A. 30 ?g human ESC whole cell lysate/extract10% SDS-PAGENANOG antibody [GT3312] (GTX627421) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse ESC 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50