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- Antibody Data
- Antigen structure
- References [105]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- AF1997 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nanog Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
ABZ92376- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.
The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient.
Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients.
Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype.
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.
SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma.
Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A.
Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development.
yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs.
Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis.
Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
DMSO increases efficiency of genome editing at two non-coding loci.
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids.
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing.
TFAP2C regulates transcription in human naive pluripotency by opening enhancers.
Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions.
Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing.
Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation.
Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease.
Generation of gene-corrected iPSC line from Parkinson's disease patient iPSC line with alpha-SNCA A53T mutation.
Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo.
High-efficiency RNA-based reprogramming of human primary fibroblasts.
Generation of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts.
Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation.
Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A.
Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells.
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status.
Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.
Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines.
TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation.
Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD).
Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal Strengths and Limitations of the Teratoma Assay and In Vitro Pluripotency Assays.
Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.
Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration.
A Comprehensive TALEN-Based Knockout Library for Generating Human-Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases.
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.
Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS).
Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells.
A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling.
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells.
A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity.
Characterization of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.
An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts.
Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application.
Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient.
Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells.
Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow.
Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization.
A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients.
Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport.
Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.
β-Catenin and NF-κB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.
Metabolic rescue in pluripotent cells from patients with mtDNA disease.
Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome.
Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
Epigenetic reprogramming induces the expansion of cord blood stem cells.
Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
Sox2 promotes tamoxifen resistance in breast cancer cells.
Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells.
A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line.
The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients.
A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells.
Differential L1 regulation in pluripotent stem cells of humans and apes.
Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome.
Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
Sox2 expression in breast tumours and activation in breast cancer stem cells.
Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells.
Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
Pedigreed primate embryonic stem cells express homogeneous familial gene profiles.
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells.
Isolation of a novel population of multipotent adult stem cells from human hair follicles.
Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.
Monitoring differentiation of human embryonic stem cells using real-time PCR.
Wang X, Liao T, Wan C, Yang X, Zhao J, Fu R, Yao Z, Huang Y, Shi Y, Chang G, Zheng Y, Luo F, Liu Z, Wang Y, Mao X, Zhao XY
PeerJ 2019;6:e6143
PeerJ 2019;6:e6143
The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient.
Tsurumi F, Baba S, Yoshinaga D, Umeda K, Hirata T, Takita J, Heike T
PloS one 2019;14(3):e0213768
PloS one 2019;14(3):e0213768
Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients.
Elkouris M, Kouroupi G, Vourvoukelis A, Papagiannakis N, Kaltezioti V, Matsas R, Stefanis L, Xilouri M, Politis PK
Frontiers in cellular neuroscience 2019;13:58
Frontiers in cellular neuroscience 2019;13:58
Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype.
Kolundzic N, Khurana P, Hobbs C, Rogar M, Ropret S, Törmä H, Ilic D, Liovic M
Stem cell research 2019 May;37:101424
Stem cell research 2019 May;37:101424
CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons.
Calatayud C, Carola G, Fernández-Carasa I, Valtorta M, Jiménez-Delgado S, Díaz M, Soriano-Fradera J, Cappelletti G, García-Sancho J, Raya Á, Consiglio A
Scientific reports 2019 May 2;9(1):6811
Scientific reports 2019 May 2;9(1):6811
SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma.
Sherman-Samis M, Onallah H, Holth A, Reich R, Davidson B
Gynecologic oncology 2019 Jun;153(3):651-660
Gynecologic oncology 2019 Jun;153(3):651-660
Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A.
Kolundzic N, Khurana P, Devito L, Donne M, Hobbs C, Jeriha J, Wong XFCC, Common JAE, Lane EB, Dubrac S, Gruber R, Schmuth M, Mauro TM, Ilic D
Stem cell research 2019 Jul;38:101462
Stem cell research 2019 Jul;38:101462
Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development.
Wang L, De Solis AJ, Goffer Y, Birkenbach KE, Engle SE, Tanis R, Levenson JM, Li X, Rausch R, Purohit M, Lee JY, Tan J, De Rosa MC, Doege CA, Aaron HL, Martins GJ, Brüning JC, Egli D, Costa R, Berbari N, Leibel RL, Stratigopoulos G
JCI insight 2019 Feb 7;4(3)
JCI insight 2019 Feb 7;4(3)
yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs.
Frank S, Ahuja G, Bartsch D, Russ N, Yao W, Kuo JC, Derks JP, Akhade VS, Kargapolova Y, Georgomanolis T, Messling JE, Gramm M, Brant L, Rehimi R, Vargas NE, Kuroczik A, Yang TP, Sahito RGA, Franzen J, Hescheler J, Sachinidis A, Peifer M, Rada-Iglesias A, Kanduri M, Costa IG, Kanduri C, Papantonis A, Kurian L
Cell stem cell 2019 Feb 7;24(2):318-327.e8
Cell stem cell 2019 Feb 7;24(2):318-327.e8
Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis.
Neri T, Hiriart E, van Vliet PP, Faure E, Norris RA, Farhat B, Jagla B, Lefrancois J, Sugi Y, Moore-Morris T, Zaffran S, Faustino RS, Zambon AC, Desvignes JP, Salgado D, Levine RA, de la Pompa JL, Terzic A, Evans SM, Markwald R, Pucéat M
Nature communications 2019 Apr 26;10(1):1929
Nature communications 2019 Apr 26;10(1):1929
Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
Shafa M, Yang F, Fellner T, Rao MS, Baghbaderani BA
Frontiers in medicine 2018;5:69
Frontiers in medicine 2018;5:69
DMSO increases efficiency of genome editing at two non-coding loci.
Stratigopoulos G, De Rosa MC, LeDuc CA, Leibel RL, Doege CA
PloS one 2018;13(6):e0198637
PloS one 2018;13(6):e0198637
3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.
Zhang Y, Yang Y, Jiang M, Huang SX, Zhang W, Al Alam D, Danopoulos S, Mori M, Chen YW, Balasubramanian R, Chuva de Sousa Lopes SM, Serra C, Bialecka M, Kim E, Lin S, Toste de Carvalho ALR, Riccio PN, Cardoso WV, Zhang X, Snoeck HW, Que J
Cell stem cell 2018 Oct 4;23(4):516-529.e5
Cell stem cell 2018 Oct 4;23(4):516-529.e5
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Kaur S, Abu-Shahba AG, Paananen RO, Hongisto H, Hiidenmaa H, Skottman H, Seppänen-Kaijansinkko R, Mannerström B
Scientific reports 2018 Oct 19;8(1):15503
Scientific reports 2018 Oct 19;8(1):15503
WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids.
Yoney A, Etoc F, Ruzo A, Carroll T, Metzger JJ, Martyn I, Li S, Kirst C, Siggia ED, Brivanlou AH
eLife 2018 Oct 12;7
eLife 2018 Oct 12;7
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W, Li T
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing.
Jeon H, Kim JY, Choi JK, Han E, Song CL, Lee J, Cho YS
Journal of clinical medicine 2018 Nov 20;7(11)
Journal of clinical medicine 2018 Nov 20;7(11)
TFAP2C regulates transcription in human naive pluripotency by opening enhancers.
Pastor WA, Liu W, Chen D, Ho J, Kim R, Hunt TJ, Lukianchikov A, Liu X, Polo JM, Jacobsen SE, Clark AT
Nature cell biology 2018 May;20(5):553-564
Nature cell biology 2018 May;20(5):553-564
Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions.
Jangid RK, Kelkar A, Muley VY, Galande S
BMC genomics 2018 May 2;19(1):313
BMC genomics 2018 May 2;19(1):313
Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing.
Xu G, Guo D, Wu F, Abbas N, Lai K, Yuan F, You K, Liu Y, Zhuang Y, Wu Y, Xu Y, Chen Y, Yang F, Pan T, Li YX
Stem cell research 2018 Mar;27:38-41
Stem cell research 2018 Mar;27:38-41
Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation.
Chang CW, Chang CC, Hsia KC, Tsai SY
Stem cell research 2018 Mar;27:21-24
Stem cell research 2018 Mar;27:21-24
Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease.
Lehtonen Š, Höytyläinen I, Voutilainen J, Sonninen TM, Kuusisto J, Laakso M, Hämäläinen RH, Oksanen M, Koistinaho J
Stem cell research 2018 Jul;30:96-99
Stem cell research 2018 Jul;30:96-99
Generation of gene-corrected iPSC line from Parkinson's disease patient iPSC line with alpha-SNCA A53T mutation.
Lee SY, Jeong S, Kim J, Chung SK
Stem cell research 2018 Jul;30:145-149
Stem cell research 2018 Jul;30:145-149
Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo.
Russell OM, Fruh I, Rai PK, Marcellin D, Doll T, Reeve A, Germain M, Bastien J, Rygiel KA, Cerino R, Sailer AW, Lako M, Taylor RW, Mueller M, Lightowlers RN, Turnbull DM, Helliwell SB
Scientific reports 2018 Jan 29;8(1):1799
Scientific reports 2018 Jan 29;8(1):1799
High-efficiency RNA-based reprogramming of human primary fibroblasts.
Kogut I, McCarthy SM, Pavlova M, Astling DP, Chen X, Jakimenko A, Jones KL, Getahun A, Cambier JC, Pasmooij AMG, Jonkman MF, Roop DR, Bilousova G
Nature communications 2018 Feb 21;9(1):745
Nature communications 2018 Feb 21;9(1):745
Generation of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts.
Erdlenbruch F, Rohwedel J, Ralfs P, Thomitzek A, Kramer J, Cakiroglu F
Stem cell research 2018 Dec;33:79-82
Stem cell research 2018 Dec;33:79-82
Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation.
Yang PJ, Yuan WX, Liu J, Li JY, Tan B, Qiu C, Zhu XL, Qiu C, Lai DM, Guo LH, Yu LY
Acta pharmacologica Sinica 2018 Aug;39(8):1305-1316
Acta pharmacologica Sinica 2018 Aug;39(8):1305-1316
Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A.
Devito L, Donne M, Kolundzic N, Khurana P, Hobbs C, Kaddour G, Dubrac S, Gruber R, Schmuth M, Mauro T, Ilic D
Stem cell research 2018 Aug;31:122-126
Stem cell research 2018 Aug;31:122-126
Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells.
Barakat TS, Halbritter F, Zhang M, Rendeiro AF, Perenthaler E, Bock C, Chambers I
Cell stem cell 2018 Aug 2;23(2):276-288.e8
Cell stem cell 2018 Aug 2;23(2):276-288.e8
Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
Wang P, Wan WW, Xiong SL, Feng H, Wu N
Cell death discovery 2017;3:16105
Cell death discovery 2017;3:16105
Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status.
Miyagoe-Suzuki Y, Nishiyama T, Nakamura M, Narita A, Takemura F, Masuda S, Minami N, Murayama K, Komaki H, Goto YI, Takeda S
Stem cells international 2017;2017:7906843
Stem cells international 2017;2017:7906843
Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.
Thomas CA, Tejwani L, Trujillo CA, Negraes PD, Herai RH, Mesci P, Macia A, Crow YJ, Muotri AR
Cell stem cell 2017 Sep 7;21(3):319-331.e8
Cell stem cell 2017 Sep 7;21(3):319-331.e8
Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines.
Miquel-Serra L, Duarri A, Muñoz Y, Kuebler B, Aran B, Costa C, Martí M, Comabella M, Malhotra S, Montalban X, Veiga A, Raya A
Stem cell research 2017 Oct;24:155-159
Stem cell research 2017 Oct;24:155-159
TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation.
Yahata N, Matsumoto Y, Omi M, Yamamoto N, Hata R
Scientific reports 2017 Nov 14;7(1):15557
Scientific reports 2017 Nov 14;7(1):15557
Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD).
Varga E, Nemes C, Bock I, Táncos Z, Berzsenyi S, Lévay G, Román V, Kobolák J, Dinnyés A
Stem cell research 2017 May;21:19-22
Stem cell research 2017 May;21:19-22
Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal Strengths and Limitations of the Teratoma Assay and In Vitro Pluripotency Assays.
Bouma MJ, van Iterson M, Janssen B, Mummery CL, Salvatori DCF, Freund C
Stem cell reports 2017 May 9;8(5):1340-1353
Stem cell reports 2017 May 9;8(5):1340-1353
Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.
Lorenz C, Lesimple P, Bukowiecki R, Zink A, Inak G, Mlody B, Singh M, Semtner M, Mah N, Auré K, Leong M, Zabiegalov O, Lyras EM, Pfiffer V, Fauler B, Eichhorst J, Wiesner B, Huebner N, Priller J, Mielke T, Meierhofer D, Izsvák Z, Meier JC, Bouillaud F, Adjaye J, Schuelke M, Wanker EE, Lombès A, Prigione A
Cell stem cell 2017 May 4;20(5):659-674.e9
Cell stem cell 2017 May 4;20(5):659-674.e9
Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration.
Saini JS, Corneo B, Miller JD, Kiehl TR, Wang Q, Boles NC, Blenkinsop TA, Stern JH, Temple S
Cell stem cell 2017 May 4;20(5):635-647.e7
Cell stem cell 2017 May 4;20(5):635-647.e7
A Comprehensive TALEN-Based Knockout Library for Generating Human-Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases.
Karakikes I, Termglinchan V, Cepeda DA, Lee J, Diecke S, Hendel A, Itzhaki I, Ameen M, Shrestha R, Wu H, Ma N, Shao NY, Seeger T, Woo N, Wilson KD, Matsa E, Porteus MH, Sebastiano V, Wu JC
Circulation research 2017 May 12;120(10):1561-1571
Circulation research 2017 May 12;120(10):1561-1571
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.
Xu Y, Wu Y, Guo D, Gao G, Lai K, Yang F, Wang K, Wu H, Lai L, Li J, Xu K, Li YX
Stem cell research 2017 Jul;22:29-32
Stem cell research 2017 Jul;22:29-32
Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS).
Kuebler B, Aran B, Miquel-Serra L, Muñoz Y, Ars E, Bullich G, Furlano M, Torra R, Marti M, Veiga A, Raya A
Stem cell research 2017 Dec;25:291-295
Stem cell research 2017 Dec;25:291-295
Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells.
Baljinnyam E, Venkatesh S, Gordan R, Mareedu S, Zhang J, Xie LH, Azzam EI, Suzuki CK, Fraidenraich D
Physiological reports 2017 Aug;5(15)
Physiological reports 2017 Aug;5(15)
A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling.
Klein S, Dvornik JL, Yarrabothula AR, Schaniel C
Stem cell research 2017 Aug;23:73-76
Stem cell research 2017 Aug;23:73-76
Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.
Sampaziotis F, de Brito MC, Geti I, Bertero A, Hannan NR, Vallier L
Nature protocols 2017 Apr;12(4):814-827
Nature protocols 2017 Apr;12(4):814-827
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells.
Ahmadian Baghbaderani B, Tian X, Scotty Cadet J, Shah K, Walde A, Tran H, Kovarcik DP, Clarke D, Fellner T
PloS one 2016;11(9):e0161229
PloS one 2016;11(9):e0161229
A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity.
Guillevic O, Ferratge S, Pascaud J, Driancourt C, Boyer-Di-Ponio J, Uzan G
PloS one 2016;11(4):e0152993
PloS one 2016;11(4):e0152993
Characterization of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.
Eguizabal C, Herrera L, De Oñate L, Montserrat N, Hajkova P, Izpisua Belmonte JC
Stem cells (Dayton, Ohio) 2016 Sep;34(9):2418-28
Stem cells (Dayton, Ohio) 2016 Sep;34(9):2418-28
An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts.
Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT
Stem cell research 2016 Sep;17(2):444-447
Stem cell research 2016 Sep;17(2):444-447
Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application.
De Sousa PA, Downie JM, Tye BJ, Bruce K, Dand P, Dhanjal S, Serhal P, Harper J, Turner M, Bateman M
Stem cell research 2016 Sep;17(2):379-390
Stem cell research 2016 Sep;17(2):379-390
Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient.
Liu Z, Zhao R
Stem cell research 2016 Sep;17(2):289-291
Stem cell research 2016 Sep;17(2):289-291
Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells.
Liu Z, Hui Y, Shi L, Chen Z, Xu X, Chi L, Fan B, Fang Y, Liu Y, Ma L, Wang Y, Xiao L, Zhang Q, Jin G, Liu L, Zhang X
Stem cell reports 2016 Sep 13;7(3):496-507
Stem cell reports 2016 Sep 13;7(3):496-507
Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow.
Mennan C, Brown S, McCarthy H, Mavrogonatou E, Kletsas D, Garcia J, Balain B, Richardson J, Roberts S
FEBS open bio 2016 Nov;6(11):1054-1066
FEBS open bio 2016 Nov;6(11):1054-1066
Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization.
Stanslowsky N, Reinhardt P, Glass H, Kalmbach N, Naujock M, Hensel N, Lübben V, Pal A, Venneri A, Lupo F, De Franceschi L, Claus P, Sterneckert J, Storch A, Hermann A, Wegner F
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Nov 23;36(47):12027-12043
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Nov 23;36(47):12027-12043
A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients.
Sun Y, Paşca SP, Portmann T, Goold C, Worringer KA, Guan W, Chan KC, Gai H, Vogt D, Chen YJ, Mao R, Chan K, Rubenstein JL, Madison DV, Hallmayer J, Froehlich-Santino WM, Bernstein JA, Dolmetsch RE
eLife 2016 Jul 26;5
eLife 2016 Jul 26;5
Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
Zeltner N, Fattahi F, Dubois NC, Saurat N, Lafaille F, Shang L, Zimmer B, Tchieu J, Soliman MA, Lee G, Casanova JL, Studer L
Nature medicine 2016 Dec;22(12):1421-1427
Nature medicine 2016 Dec;22(12):1421-1427
V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
Pino-Barrio MJ, García-García E, Menéndez P, Martínez-Serrano A
PloS one 2015;10(3):e0118499
PloS one 2015;10(3):e0118499
GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport.
Freibaum BD, Lu Y, Lopez-Gonzalez R, Kim NC, Almeida S, Lee KH, Badders N, Valentine M, Miller BL, Wong PC, Petrucelli L, Kim HJ, Gao FB, Taylor JP
Nature 2015 Sep 3;525(7567):129-33
Nature 2015 Sep 3;525(7567):129-33
Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.
Francescangeli F, Contavalli P, De Angelis ML, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro NP, Watanabe K, Salomon DS, De Maria R, Minchiotti G, Zeuner A
Cell death and differentiation 2015 Oct;22(10):1700-13
Cell death and differentiation 2015 Oct;22(10):1700-13
β-Catenin and NF-κB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.
Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, Chi L, Filion LG, Figeys D, Wang L
Cell death and differentiation 2015 Feb;22(2):298-310
Cell death and differentiation 2015 Feb;22(2):298-310
Metabolic rescue in pluripotent cells from patients with mtDNA disease.
Ma H, Folmes CD, Wu J, Morey R, Mora-Castilla S, Ocampo A, Ma L, Poulton J, Wang X, Ahmed R, Kang E, Lee Y, Hayama T, Li Y, Van Dyken C, Gutierrez NM, Tippner-Hedges R, Koski A, Mitalipov N, Amato P, Wolf DP, Huang T, Terzic A, Laurent LC, Izpisua Belmonte JC, Mitalipov S
Nature 2015 Aug 13;524(7564):234-8
Nature 2015 Aug 13;524(7564):234-8
Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
Liu L, Wu L, Wei X, Ling J
Stem cells and development 2015 Apr 15;24(8):962-72
Stem cells and development 2015 Apr 15;24(8):962-72
Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome.
Kobayashi J, Yoshida M, Tarui S, Hirata M, Nagai Y, Kasahara S, Naruse K, Ito H, Sano S, Oh H
PloS one 2014;9(7):e102796
PloS one 2014;9(7):e102796
Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
Bayin NS, Modrek AS, Dietrich A, Lebowitz J, Abel T, Song HR, Schober M, Zagzag D, Buchholz CJ, Chao MV, Placantonakis DG
PloS one 2014;9(12):e116114
PloS one 2014;9(12):e116114
Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
Sproul AA, Jacob S, Pre D, Kim SH, Nestor MW, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele JW, Kahler DJ, Dranovsky A, Arancio O, Crary JF, Gandy S, Noggle SA
PloS one 2014;9(1):e84547
PloS one 2014;9(1):e84547
Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
Stem cells and development 2014 Sep 15;23(18):2225-36
Stem cells and development 2014 Sep 15;23(18):2225-36
Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
Bershteyn M, Hayashi Y, Desachy G, Hsiao EC, Sami S, Tsang KM, Weiss LA, Kriegstein AR, Yamanaka S, Wynshaw-Boris A
Nature 2014 Mar 6;507(7490):99-103
Nature 2014 Mar 6;507(7490):99-103
Epigenetic reprogramming induces the expansion of cord blood stem cells.
Chaurasia P, Gajzer DC, Schaniel C, D'Souza S, Hoffman R
The Journal of clinical investigation 2014 Jun;124(6):2378-95
The Journal of clinical investigation 2014 Jun;124(6):2378-95
Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
Piao Y, Hung SS, Lim SY, Wong RC, Ko MS
Stem cells translational medicine 2014 Jul;3(7):787-91
Stem cells translational medicine 2014 Jul;3(7):787-91
Sox2 promotes tamoxifen resistance in breast cancer cells.
Piva M, Domenici G, Iriondo O, Rábano M, Simões BM, Comaills V, Barredo I, López-Ruiz JA, Zabalza I, Kypta R, Vivanco Md
EMBO molecular medicine 2014 Jan;6(1):66-79
EMBO molecular medicine 2014 Jan;6(1):66-79
Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
Luo L, Kawakatsu M, Guo CW, Urata Y, Huang WJ, Ali H, Doi H, Kitajima Y, Tanaka T, Goto S, Ono Y, Xin HB, Hamano K, Li TS
Scientific reports 2014 Jan 21;4:3779
Scientific reports 2014 Jan 21;4:3779
Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells.
Nathwani BB, Miller CH, Yang TL, Solimano JL, Liao JC
Stem cells and development 2014 Jan 15;23(2):115-23
Stem cells and development 2014 Jan 15;23(2):115-23
A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima KJ, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K
Stem cells translational medicine 2014 Aug;3(8):911-22
Stem cells translational medicine 2014 Aug;3(8):911-22
Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line.
Wu YL, Ma SR, Peng T, Teng ZH, Liang XY, Guo GZ, Zhang HF, Li KC
International journal of molecular sciences 2014 Aug 14;15(8):14180-90
International journal of molecular sciences 2014 Aug 14;15(8):14180-90
The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients.
Fatima A, Kaifeng S, Dittmann S, Xu G, Gupta MK, Linke M, Zechner U, Nguemo F, Milting H, Farr M, Hescheler J, Sarić T
PloS one 2013;8(12):e83005
PloS one 2013;8(12):e83005
A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells.
Zhang R, Mjoseng HK, Hoeve MA, Bauer NG, Pells S, Besseling R, Velugotla S, Tourniaire G, Kishen RE, Tsenkina Y, Armit C, Duffy CR, Helfen M, Edenhofer F, de Sousa PA, Bradley M
Nature communications 2013;4:1335
Nature communications 2013;4:1335
Differential L1 regulation in pluripotent stem cells of humans and apes.
Marchetto MCN, Narvaiza I, Denli AM, Benner C, Lazzarini TA, Nathanson JL, Paquola ACM, Desai KN, Herai RH, Weitzman MD, Yeo GW, Muotri AR, Gage FH
Nature 2013 Nov 28;503(7477):525-529
Nature 2013 Nov 28;503(7477):525-529
Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome.
Bellin M, Casini S, Davis RP, D'Aniello C, Haas J, Ward-van Oostwaard D, Tertoolen LG, Jung CB, Elliott DA, Welling A, Laugwitz KL, Moretti A, Mummery CL
The EMBO journal 2013 Dec 11;32(24):3161-75
The EMBO journal 2013 Dec 11;32(24):3161-75
Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes SM, De Sutter P
Stem cells and development 2013 Dec 1;22(23):3141-55
Stem cells and development 2013 Dec 1;22(23):3141-55
Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF
Cell stem cell 2013 Aug 1;13(2):246-54
Cell stem cell 2013 Aug 1;13(2):246-54
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
Hayashi R, Ishikawa Y, Ito M, Kageyama T, Takashiba K, Fujioka T, Tsujikawa M, Miyoshi H, Yamato M, Nakamura Y, Nishida K
PloS one 2012;7(9):e45435
PloS one 2012;7(9):e45435
The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
Pekkanen-Mattila M, Ojala M, Kerkelä E, Rajala K, Skottman H, Aalto-Setälä K
Stem cells international 2012;2012:875059
Stem cells international 2012;2012:875059
Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
Kim SY, Kim MJ, Jung H, Kim WK, Kwon SO, Son MJ, Jang IS, Choi JS, Park SG, Park BC, Han YM, Lee SC, Cho YS, Bae KH
Stem cells and development 2012 May 20;21(8):1272-86
Stem cells and development 2012 May 20;21(8):1272-86
Sox2 expression in breast tumours and activation in breast cancer stem cells.
Leis O, Eguiara A, Lopez-Arribillaga E, Alberdi MJ, Hernandez-Garcia S, Elorriaga K, Pandiella A, Rezola R, Martin AG
Oncogene 2012 Mar 15;31(11):1354-65
Oncogene 2012 Mar 15;31(11):1354-65
Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
Stem cells and development 2012 Feb 10;21(3):404-10
Stem cells and development 2012 Feb 10;21(3):404-10
A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
Stem cells (Dayton, Ohio) 2011 Oct;29(10):1517-27
Stem cells (Dayton, Ohio) 2011 Oct;29(10):1517-27
CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
Stem cells (Dayton, Ohio) 2011 Apr;29(4):609-17
Stem cells (Dayton, Ohio) 2011 Apr;29(4):609-17
Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
Carvajal-Vergara X, Sevilla A, D'Souza SL, Ang YS, Schaniel C, Lee DF, Yang L, Kaplan AD, Adler ED, Rozov R, Ge Y, Cohen N, Edelmann LJ, Chang B, Waghray A, Su J, Pardo S, Lichtenbelt KD, Tartaglia M, Gelb BD, Lemischka IR
Nature 2010 Jun 10;465(7299):808-12
Nature 2010 Jun 10;465(7299):808-12
Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
Chayosumrit M, Tuch B, Sidhu K
Biomaterials 2010 Jan;31(3):505-14
Biomaterials 2010 Jan;31(3):505-14
SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
Chng Z, Teo A, Pedersen RA, Vallier L
Cell stem cell 2010 Jan 8;6(1):59-70
Cell stem cell 2010 Jan 8;6(1):59-70
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL
Nature methods 2010 Dec;7(12):989-94
Nature methods 2010 Dec;7(12):989-94
Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J
PloS one 2010 Dec 30;5(12):e14397
PloS one 2010 Dec 30;5(12):e14397
Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells.
Chang CW, Lai YS, Pawlik KM, Liu K, Sun CW, Li C, Schoeb TR, Townes TM
Stem cells (Dayton, Ohio) 2009 May;27(5):1042-9
Stem cells (Dayton, Ohio) 2009 May;27(5):1042-9
Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K
FEBS letters 2009 Mar 18;583(6):1029-33
FEBS letters 2009 Mar 18;583(6):1029-33
Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9826-30
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9826-30
Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
FEBS letters 2009 Feb 4;583(3):561-7
FEBS letters 2009 Feb 4;583(3):561-7
Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
Biotechnology and bioengineering 2009 Apr 15;102(6):1636-44
Biotechnology and bioengineering 2009 Apr 15;102(6):1636-44
The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
Zuk PA
Journal of cellular biochemistry 2009 Apr 1;106(5):867-77
Journal of cellular biochemistry 2009 Apr 1;106(5):867-77
High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
Cell stem cell 2008 Jun 5;2(6):602-12
Cell stem cell 2008 Jun 5;2(6):602-12
Pedigreed primate embryonic stem cells express homogeneous familial gene profiles.
Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G
Stem cells (Dayton, Ohio) 2007 Nov;25(11):2695-2704
Stem cells (Dayton, Ohio) 2007 Nov;25(11):2695-2704
Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
Cell 2007 Nov 30;131(5):861-72
Cell 2007 Nov 30;131(5):861-72
Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells.
Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL, Chang YJ
Biology of reproduction 2006 Mar;74(3):545-51
Biology of reproduction 2006 Mar;74(3):545-51
Isolation of a novel population of multipotent adult stem cells from human hair follicles.
Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X
The American journal of pathology 2006 Jun;168(6):1879-88
The American journal of pathology 2006 Jun;168(6):1879-88
Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.
Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M
Stem cells (Dayton, Ohio) 2005 Sep;23(8):1035-43
Stem cells (Dayton, Ohio) 2005 Sep;23(8):1035-43
Monitoring differentiation of human embryonic stem cells using real-time PCR.
Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P
Stem cells (Dayton, Ohio) 2005 Nov-Dec;23(10):1460-7
Stem cells (Dayton, Ohio) 2005 Nov-Dec;23(10):1460-7
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Supportive validation
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- Main image
- Experimental details
- Detection of Human Nanog by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and Tera-2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
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- Main image
- Experimental details
- Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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- R&D Systems (provider)
- Main image
- Experimental details
- Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
