AP12635b

antibody from Abgent

Targeting: CYP2S1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12635b

Provider

Abgent

Proper citation

Abgent Cat#AP12635b, RRID:AB_10818751

Product name

CYP2S1 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysate from BEAS 2B cell line, using CYP2S1 Antibody (C-term)(Cat. #AP12635b). AP12635b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CYP2S1 Antibody (C-term) (Cat. #AP12635b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CYP2S1 antibody detected the CYP2S1 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Confocal immunofluorescent analysis of CYP2S1 Antibody (C-term)(Cat#AP12635b) with HepG2 cell followed by Alexa Fluor4d?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). "

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

CYP2S1 Antibody (C-term) (Cat. #AP12635b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2S1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50