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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-17073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAS Monoclonal Antibody (4F8H6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17073 targets FAS in FACS, ICC, IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4F8H6
- Concentration
- 1 mg/mL
Submitted references Gene therapy for human ovarian cancer cells using efficient expression of Fas gene combined with γδT cells.
Lin J, Zeng D, He H, Tan G, Lan Y, Jiang F, Sheng S
Molecular medicine reports 2017 Oct;16(4):3791-3798
Molecular medicine reports 2017 Oct;16(4):3791-3798
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD95/Fas using a CD95/Fas monoclonal antibody (Product # MA5-17073) against a human FAS (AA: 87-278) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD95/Fas using CD95/Fas monoclonal antibody (Product # MA5-17073) in HeLa (1), Jurkat (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD95/Fas using a CD95/Fas monoclonal antibody (Product # MA5-17073) against a human FAS (AA: 87-278) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD95/Fas using CD95/Fas monoclonal antibody (Product # MA5-17073) in HeLa (1), Jurkat (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-FAS Monoclonal Antibody (4F8H6) (Product # MA5-17073) and a 16 kDa band corresponding to an isoform of FAS (Tumor necrosis factor receptor superfamily member 6) was observed across cell lines tested except in HUVEC and MDA-MB-231 which are reported low expressing for the protein. Whole cell extracts (40 µg lysate) of Jurkat (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), HUVEC (Lane 4), MDA-MB-231 (Lane 5), NK-92 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CD95/Fas monoclonal antibody (Product # MA5-17073) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using CD95/Fas monoclonal antibody (Product # MA5-17073) (green) and negative control (red).
