Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A00055 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Fas Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Fas detection. Tested with WB, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Regulatory role of oxidative stress in retrorsine - Induced apoptosis and autophagy in primary rat hepatocytes.
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.
Perfluorooctanoic acid exposure in early pregnancy induces oxidative stress in mice uterus and liver.
Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.
Zhu Y, Zhang S, Shao Y, Tang L, Zhang C, Tang S, Lu H
Ecotoxicology and environmental safety 2024 Jul 1;279:116515
Ecotoxicology and environmental safety 2024 Jul 1;279:116515
Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line.
Wang H, Liu Y, Guo Z, Wu K, Zhang Y, Tian Y, Zhao B, Lu H
Research in veterinary science 2022 Mar;143:124-133
Research in veterinary science 2022 Mar;143:124-133
Perfluorooctanoic acid exposure in early pregnancy induces oxidative stress in mice uterus and liver.
Zhang Y, Zhang L, Bao J, Liu L, Wang X
Environmental science and pollution research international 2021 Dec;28(46):66355-66365
Environmental science and pollution research international 2021 Dec;28(46):66355-66365
Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation.
Gao K, Chen Y, Wei L, Li S, Jin X, Cong C, Yuan Y, Long D, Li Y, Cheng J, Lu Y
Cell biochemistry and function 2008 Dec;26(8):900-7
Cell biochemistry and function 2008 Dec;26(8):900-7
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Fas using anti-Fas antibody (A00055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fas antigen affinity purified polyclonal antibody (Catalog # A00055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fas at approximately 37KD. The expected band size for Fas is at 37KD.
- Additional image
