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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA501237 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501237, RRID:AB_11126957
- Product name
- Anti-BCL10 mouse monoclonal antibody, clone OTI4A8 (formerly 4A8)
- Antibody type
- Monoclonal
- Description
- Anti-BCL10 mouse monoclonal antibody, clone OTI4A8 (formerly 4A8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BCL10
- Isotype
- IgG
- Antibody clone number
- OTI4A8
- Vial size
- 100 µl
- Concentration
- 0.73 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10 .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BCL10 mouse monoclonal antibody (TA501237) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL10 (RC208752 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501237, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501237, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCL10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501237, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-BCL10 antibody(TA501237),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
