Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-804-134-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-134-C100, RRID:AB_2050803
- Product name
- Bcl-10 monoclonal antibody (Carmen-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgM
- Antibody clone number
- Carmen-1
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of mouse and human Bcl-10. Lane 1: control, mock-transfected HEK 293 T cells. Lane 2: mouse Bcl-10-transfected HEK 293 T cells. Lane 3: human Bcl-10-transfected HEK 293 T cells. Method: Cell extracts from HEK 293 T cells (5x103) transfected with mouse and human Bcl-10-expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with MAb to Bcl-10 (Carmen-1) (Prod. No. ALX-804-134)Êat 1µg/ml. Proteins were visualized using a proxidase-conjugated antibody to rat IgM (Prod. No. ALX-211-055) and a chemiluminescence detection system.