- MA1-23160 - Invitrogen Antibodies BRCA1 antibody

Submitted references Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis.

Fukushima K, Satoh T, Sugihara F, Sato Y, Okamoto T, Mitsui Y, Yoshio S, Li S, Nojima S, Motooka D, Nakamura S, Kida H, Standley DM, Morii E, Kanto T, Yanagita M, Matsuura Y, Nagasawa T, Kumanogoh A, Akira S
Immunity 2020 Mar 17;52(3):542-556.e13

Premature polyadenylation of MAGI3 is associated with diminished N(6)-methyladenosine in its large internal exon.

Ni TK, Elman JS, Jin DX, Gupta PB, Kuperwasser C
Scientific reports 2018 Jan 23;8(1):1415

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BRCA1 using 30 µg of A) 293T and B) HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a BRCA1 monoclonal antibody (Product # MA1-23160) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of BRCA1 in MCF-7 and MEF (mouse embryonic fibroblast) cell lysate. Samples were probed with a BRCA1 monoclonal antibody (Product # MA1-23160) at a dilution of 1:1000 with a 5-15% gradient gel.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BRCA1 Monoclonal Antibody (17F8) detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and blotted with BRCA1 Monoclonal Antibody (17F8) (Product # MA1-23160) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of BRCA1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR813233_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of BRCA1 was performed by loading 60 µg of HeLa Cas9 (Lane 1) andHeLa BRCA1 KO (Lane 2) modified whole cell extracts. The samples were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-BRCA1 Monoclonal Antibody (17F8) (Product # MA1-23160, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5000 dilution) using the iBright™ FL 1500 (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to BRCA1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BRCA1 antibody 6B4 (Product # MA1-23164) and BRCA1 antibody 17F8 (Product # MA1-23160) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: BRCA1 antibody 6B4 (Product # MA1-23164) and BRCA1 antibody 17F8 (Product # MA1-23160) was used for IP-Western Blot assay. 6B4 alone (4 µg), 17F8 alone (4 µg), 6B4 plus 17F8 (2 µg each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in Western Blot using BRCA1 antibody 6B4 at 1:1,000 dilution.

MA1-23160

Antibody data