NBP2-03620

antibody from Novus Biologicals

Targeting: PRKCE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03620

Provider

Novus Biologicals

Product name

Mouse Monoclonal PKC epsilon Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKC epsilon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PKC epsilon Antibody (4G8) [NBP2-03620] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PKC epsilon monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKC epsilon mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKC epsilon mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymph node tissue using anti-PKC epsilon mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKC epsilon mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PKC epsilon Antibody (4G8) [NBP2-03620] - Staining of paraffin-embedded Human prostate tissue using anti-PKC epsilon mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PKC epsilon Antibody (4G8) [NBP2-03620] - Analysis of Hela cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).