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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2776 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2776, RRID:AB_1572128
- Product name
- CASK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CASK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mapping and expression analysis of the human CASK gene.
Human CASK/LIN-2 binds syndecan-2 and protein 4.1 and localizes to the basolateral membrane of epithelial cells.
Crystal structure of the hCASK PDZ domain reveals the structural basis of class II PDZ domain target recognition.
Stevenson D, Laverty HG, Wenwieser S, Douglas M, Wilson JB
Mammalian genome : official journal of the International Mammalian Genome Society 2000 Oct;11(10):934-7
Mammalian genome : official journal of the International Mammalian Genome Society 2000 Oct;11(10):934-7
Human CASK/LIN-2 binds syndecan-2 and protein 4.1 and localizes to the basolateral membrane of epithelial cells.
Cohen AR, Woods DF, Marfatia SM, Walther Z, Chishti AH, Anderson JM
The Journal of cell biology 1998 Jul 13;142(1):129-38
The Journal of cell biology 1998 Jul 13;142(1):129-38
Crystal structure of the hCASK PDZ domain reveals the structural basis of class II PDZ domain target recognition.
Daniels DL, Cohen AR, Anderson JM, Brünger AT
Nature structural biology 1998 Apr;5(4):317-25
Nature structural biology 1998 Apr;5(4):317-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASK polyclonal antibody (Cat # PAB2776) in mouse kidney lysate. CASK (arrow) was detected using purified polyclonal. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CASK polyclonal antibody (Cat # PAB2776) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
