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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-41564 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCOR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: MVEKGPEVSG KRRGRNNAAA SASAAAASAA ASAACASPAA TAASGAAASS
- Concentration
- 1 mg/mL
Submitted references Nuclear S-nitrosylation impacts tissue regeneration in zebrafish.
Matrone G, Jung SY, Choi JM, Jain A, Leung HE, Rajapakshe K, Coarfa C, Rodor J, Denvir MA, Baker AH, Cooke JP
Nature communications 2021 Nov 1;12(1):6282
Nature communications 2021 Nov 1;12(1):6282
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-RCOR1 polyclonal antibody (Product # PA5-41564).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human lung tissue using an anti-RCOR1 polyclonal antibody (Product # PA5-41564).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human lung tissue using an anti-RCOR1 polyclonal antibody (Product # PA5-41564).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Role of S-nitrosylation of Kdm1a in tailfin regeneration in adult zebrafish. A MS/MS fragmentation spectrum for the Cys334-containing peptide of Kdm1a. Peptide sequence is shown at the top left of the spectrum, with the annotation of the identified matched amino terminus-containing ions (b ions) in black and the carboxyl terminus-containing ions (y ions) in red. The spectrum confirms the identity of the peptides CPLYEAN and the labeled C as S-nitrosylated cysteine. B Line graph reporting the quantification of Kdm1a S-nitrosylation (normalized by total Kdm1a) and Kdm1a activity during tailfin regeneration. C Western blotting (WB) for S-nitrosylated Kdm1a in uninjured and at 3, 5, and 10 dpa. Samples were previously immunoprecipitated (IP) for Kdm1a. IP with IgG and input were used as controls. D - F WB for Kdm1a, CoRest and NuRD complexes components following IP with Kdm1a antibody in tailfin uninjured or injured at 5 dpa. Dpa days post-amputation. N = 3 biological replicates.
