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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PPS023 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Mouse and rat ~51 kDa GABAA R alpha 1 subunit, N-Terminus
- Reactivity
- Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of GABAA R alpha 1 N-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 1 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~51 kDa alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.
