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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB300-191 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-191, RRID:AB_2108812
- Product name
- Rabbit Polyclonal GABA-AR alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~51k alpha1-subunit of the GABAA receptor in Western blots of rat brain. Labeling is absent in alpha1-subunit knockout animals.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.15 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Strain-specific programming of prenatal ethanol exposure across generations.
Altered GABAA receptor expression and seizure threshold following acute ethanol challenge in mice lacking the RIIβ subunit of PKA.
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Ethanol Activation of Protein Kinase A Regulates GABA(A) Receptor Subunit Expression in the Cerebral Cortex and Contributes to Ethanol-Induced Hypnosis.
Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals.
Subunit Compensation and Plasticity of Synaptic GABA(A) Receptors Induced by Ethanol in α4 Subunit Knockout Mice.
Plasticity of GABAA receptors after ethanol pre-exposure in cultured hippocampal neurons.
Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons.
Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain.
Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration.
Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration.
Popoola DO, Nizhnikov ME, Cameron NM
Alcohol (Fayetteville, N.Y.) 2017 May;60:191-199
Alcohol (Fayetteville, N.Y.) 2017 May;60:191-199
Altered GABAA receptor expression and seizure threshold following acute ethanol challenge in mice lacking the RIIβ subunit of PKA.
Carlson SL, O'Buckley TK, Thomas R, Thiele TE, Morrow AL
Neurochemical research 2014 Jun;39(6):1079-87
Neurochemical research 2014 Jun;39(6):1079-87
Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes.
Mendu SK, Bhandage A, Jin Z, Birnir B
PloS one 2012;7(8):e42959
PloS one 2012;7(8):e42959
Ethanol Activation of Protein Kinase A Regulates GABA(A) Receptor Subunit Expression in the Cerebral Cortex and Contributes to Ethanol-Induced Hypnosis.
Kumar S, Ren Q, Beckley JH, O'Buckley TK, Gigante ED, Santerre JL, Werner DF, Morrow AL
Frontiers in neuroscience 2012;6:44
Frontiers in neuroscience 2012;6:44
Hispidulin inhibits the release of glutamate in rat cerebrocortical nerve terminals.
Lin TY, Lu CW, Wang CC, Lu JF, Wang SJ
Toxicology and applied pharmacology 2012 Sep 1;263(2):233-43
Toxicology and applied pharmacology 2012 Sep 1;263(2):233-43
Subunit Compensation and Plasticity of Synaptic GABA(A) Receptors Induced by Ethanol in α4 Subunit Knockout Mice.
Suryanarayanan A, Liang J, Meyer EM, Lindemeyer AK, Chandra D, Homanics GE, Sieghart W, Olsen RW, Spigelman I
Frontiers in neuroscience 2011;5:110
Frontiers in neuroscience 2011;5:110
Plasticity of GABAA receptors after ethanol pre-exposure in cultured hippocampal neurons.
Shen Y, Lindemeyer AK, Spigelman I, Sieghart W, Olsen RW, Liang J
Molecular pharmacology 2011 Mar;79(3):432-42
Molecular pharmacology 2011 Mar;79(3):432-42
Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons.
Kumar S, Suryanarayanan A, Boyd KN, Comerford CE, Lai MA, Ren Q, Morrow AL
Molecular pharmacology 2010 May;77(5):793-803
Molecular pharmacology 2010 May;77(5):793-803
Effect of chronic administration of ethanol on the regulation of tyrosine kinase phosphorylation of the GABAA receptor subunits in the rat brain.
Marutha Ravindran CR, Mehta AK, Ticku MK
Neurochemical research 2007 Jul;32(7):1179-87
Neurochemical research 2007 Jul;32(7):1179-87
Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration.
Mehta AK, Ticku MK
Brain research. Molecular brain research 1999 Apr 6;67(1):194-9
Brain research. Molecular brain research 1999 Apr 6;67(1):194-9
Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration.
Mehta AK, Ticku MK
Brain research. Molecular brain research 1999 Apr 6;67(1):194-9
Brain research. Molecular brain research 1999 Apr 6;67(1):194-9
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.
