Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PPS022 - Provider product page
- Provider
- R&D Systems
- Product name
- GABA-A R alpha 1 C-Terminus Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat and bovine ~51 kDa GABAA R alpha 1 subunit, C-Terminus
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation.
Di XJ, Wang YJ, Han DY, Fu YL, Duerfeldt AS, Blagg BS, Mu TW
The Journal of biological chemistry 2016 Apr 29;291(18):9526-39
The Journal of biological chemistry 2016 Apr 29;291(18):9526-39
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GABAA R alpha 1 C-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 1 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~51kDa alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.
