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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-119918 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GABRB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Human brian tissue lysate, rat cerebellum tissue lysate, rat brian tissue lysate, mouse brain tissue, A549.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GABRB2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. GABRB2 Polyclonal Antibody (Product # PA5-119918) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human Brain tissue lysate. Lane 2: Rat cerebellum tissue lysate. Lane 3: Rat Brain tissue lysate. Predicted band size: 59 kDa. Observed band size: 80/50/65 kDa (Glycosylation).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse brain tissue using GABRB2 Polyclonal Antibody (Product # PA5-119918). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the GABRB2 antibody at a dilution of 1:200 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of GABRB2 in A549 cells using GABRB2 Polyclonal Antibody (Product # PA5-119918) at 1 µg/mL (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at 4°C for 1 hour, the cells were stained with a Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at 4°C (dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
