AF6124

antibody from Novus Biologicals

Targeting: IZUMO1R Folbp3, FOLR4, JUNO

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6124

Provider

Novus Biologicals

Product name

Sheep Polyclonal FOLR4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse FOLR4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FOLR1, rhFOLR2, rhFOLR3, and rhFOLR4 is observed.

Reactivity

Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse FOLR4 by Western Blot. Western blot shows lysates of mouse CD⁺CD25⁺ regulatory T cells. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6124) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR4 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of FOLR4 in Mouse Splenocytes Gated on FoxP3 Cells by Flow Cytometry. Mouse splenocytes gated on FoxP3 cells treated with 10 μL/mL anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML), and Human TGF-beta 1 (Catalog # 100-B) for 3 days to induce T regulatory cells (Tregs) were stained with Sheep Anti-Mouse FOLR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6124, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3 Staining Buffer (Catalog # FC011).