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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- GTX101450 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101450, RRID:AB_2037161
- Product name
- SAFB antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Autoantibody to scaffold attachment factor B (SAFB): A novel connective tissue disease-related autoantibody associated with interstitial lung disease.
Resources for the Comprehensive Discovery of Functional RNA Elements.
Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells.
Takeuchi A, Matsushita T, Kaji K, Okamoto Y, Yasui M, Hirata M, Oishi N, Higashi A, Seishima M, Asano T, Fujimoto M, Kuwana M, Takehara K, Hamaguchi Y
Journal of autoimmunity 2017 Jan;76:101-107
Journal of autoimmunity 2017 Jan;76:101-107
Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells.
Hong E, Best A, Gautrey H, Chin J, Razdan A, Curk T, Elliott DJ, Tyson-Capper AJ
BioMed research international 2015;2015:395816
BioMed research international 2015;2015:395816
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: Hep G2 (GTX27900) 7.5% SDS PAGE GTX101450 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Scaffold attachment factor B1 antibody (GTX101450) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Scaffold attachment factor B1(GTX101450) antibody at 1:100 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.
