H00006294-M04

antibody from Abnova Corporation

Targeting: SAFB HET, SAFB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006294-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006294-M04, RRID:AB_565809

Product name

SAFB monoclonal antibody (M04), clone 5A11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SAFB.

Antigen sequence

DGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVA
DCVEDDDADNLQESLSDSRELVEGEMKELPEQLQE
HAIEDKETINNLDTSSSDFT


Isotype

IgG

Antibody clone number

5A11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SAFB monoclonal antibody (M04), clone 5A11 Western Blot analysis of SAFB expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SAFB is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SAFB on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SAFB on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol