Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5567 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse C4.4A/LYPD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse C4.4A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human C4.4A is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q91YK8
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse C4.4A/LYPD3 by Western Blot. Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5567) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for C4.4A/LYPD3 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- C4.4A/LYPD3 in Mouse Ovary. C4.4A/LYPD3 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse C4.4A/LYPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5567) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.