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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG54719 - Provider product page
- Provider
- Arigo
- Product name
- anti-Rac1 antibody
- Antibody type
- Monoclonal
- Antigen
- KLH-conjugated synthetic peptide from Human RAC1 protein (NP_008839.2).
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antibody clone number
- 1301CT276.121.104
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of A431, mouse NIH/3T3, rat C6 cell line lysates (from left to right) stained with ARG54719 anti-Rac1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human stomach section stained with ARG54719 anti-Rac1 antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human skin section stained with ARG54719 anti-Rac1 antibody at 1:25 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: U-87 MG cells stained with ARG54719 anti-Rac1 antibody (green) at 1:100 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
