20786-1-AP

antibody from Invitrogen Antibodies

Targeting: WDR55 FLJ20195, FLJ21702

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20786-1-AP

Provider

Invitrogen Antibodies

Product name

WDR55 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDMRQHEEY IADMALDPAK KLLLTASGDG CLGIFNIKRR RFELLSEPQS GDLTSVTLMK WGKKVACGSS EGTIYLFNWN GFGATSDRFA LRAESIDCMV PVTESLLCTG STDGVIRAVN ILPNRVVGSV GQHTGEPVEE LALSHCGRFL ASSGHDQRLK FWDMAQLRAV VVDDYRRRKK KGGPLRALSS KTWSTDDFFA GLREEGEDSM AQEEKEETGD DSD (162-383 aa encoded by BC002482)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20786-1-AP (WDR55 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20786-1-AP (WDR55 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20786-1-AP ( WDR55 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 20786-1-AP) (WDR55 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using WDR55 antibody 20786-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 20786-1-AP (WDR55 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 20786-1-AP (WDR55 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-WDR55 (IP:20786-1-AP, 4ug; Detection:20786-1-AP 1:300) with HeLa cells lysate 3440ug.