GTX120294

antibody from GeneTex

Targeting: RAB35 H-ray

Immunocytochemistry

Antibody data

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Validation data

RAB35 protein structure - GTX120294 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: U87-MG 12% SDS PAGE GTX120294 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.