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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-B10049 - Provider product page
- Provider
- LSBio
- Product name
- PathPlus™ NOS2 / iNOS Antibody (aa997-1058, clone 4E5) LS-B10049
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4E5
- Storage
- Short term: store at 4°C. Long term: store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using NOS2 monoclonal antibody against human NOS2 (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using NOS2 mouse monoclonal antibody against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NOS2 / iNOS antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NOS2 / iNOS antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NOS2 / iNOS antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
