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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52914PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52914PU-N, RRID:AB_11148965
- Product name
- anti NOS2 (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated corresponding to the central region (between 830-860 aa) of human NOS2A
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- Lot Specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in CEM cell line lysates (35ug/lane) using NOS2 antibody Cat.-No. AP52914PU-N. This demonstrates this antibody detected the NOS2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NOS2 with hela cells using NOS2Â antibody Cat.-No. AP52914PU-N, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis human hepato-carcinoma (Formalin-fixed, Paraffin-embedded) using NOS2 antibody Cat.-No. AP52914PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram) using NOS2Â antibody Cat.-No. AP52914PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
