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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- Ab110648 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- iNOS Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human NOS2 Antibody, Monoclonal (C1), could be used for WB, IHC and so on. Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL Protein Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 734-996- Antibody clone number
- C1
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- iNOS Mouse mAb (Ab110648) - Western Blot All lanes: iNOS Mouse mAb (Ab110648) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 131 kDa Observed band size: 118 kDa Exposure time: 6.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- iNOS Mouse mAb (Ab110648) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL iNOS Mouse mAb (Ab110648) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- iNOS Mouse mAb (Ab110648) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 10 µg/mL iNOS Mouse mAb (Ab110648) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
