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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB23503 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB23503, RRID:AB_11122204
- Product name
- ELMOD1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein corresponding to amino acids of human ELMOD1.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
MRKLTGRCELQRICYNTKPGASRTMKIETSLRDSK
SKLLQTSVSVHPDAIEKTIEDIMELKKINPDVNPQ
LGISLQACLLQIVGYRN- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ELMOD1 polyclonal antibody (Cat # PAB23503) at 1:250-1:500 dilution.
- Validation comment
- Western Blot (Transfected lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 with ELMOD1 polyclonal antibody (Cat # PAB23503) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human spleen with ELMOD1 polyclonal antibody (Cat # PAB23503) shows strong cytoplasmic positivity in cells of red pulp at 1:500-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
