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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10506 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10506, RRID:AB_11190321
- Product name
- BMI1 monoclonal antibody, clone 3E3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant BMI1.
- Isotype
- IgG
- Antibody clone number
- 3E3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against human BMI1 (aa : 1-326) recombinant protein. (Expected MW is 62.4 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human rectum cancer (B) tissues using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green) and negative control (red).
- Validation comment
- Flow Cytometry
