10832-1-AP

antibody from Proteintech Group

Targeting: BMI1 PCGF4, RNF51

Provider product page for 10832-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [20]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

Submit

Product number: 10832-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10832-1-AP, RRID:AB_2065392
Product name: BMI1 antibody
Antibody type: Polyclonal
Description: KD/KO validated BMI1 antibody (Cat. #10832-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

BMI1 protein structure - 10832-1-AP shown in red.

Submitted references miR-135a Reduces Osteosarcoma Pulmonary Metastasis by Targeting Both BMI1 and KLF4.

Chen C, Mao X, Cheng C, Jiao Y, Zhou Y, Ren T, Wu Z, Lv Z, Sun X, Guo W
Frontiers in oncology 2021;11:620295

A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.

Wenduerma, Yamada NO, Wang T, Senda T
Medical molecular morphology 2021 Sep;54(3):203-215

FOXD1 promotes EMT and cell stemness of oral squamous cell carcinoma by transcriptional activation of SNAI2.

Chen Y, Liang W, Liu K, Shang Z
Cell & bioscience 2021 Aug 4;11(1):154

CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.

Jiang H, Niu F, Zheng Y, Xu Y
Molecular medicine reports 2021 Apr;23(4)

Internal Ribosome Entry Sites Mediate Cap-Independent Translation of Bmi1 in Nasopharyngeal Carcinoma.

Wang H, Zhu Y, Hu L, Li Y, Liu G, Xia T, Xiong D, Luo Y, Liu B, An Y, Li M, Huang Y, Zhong Q, Zeng M
Frontiers in oncology 2020;10:1678

CCL21/CCR7 interaction promotes EMT and enhances the stemness of OSCC via a JAK2/STAT3 signaling pathway.

Chen Y, Shao Z, Jiang E, Zhou X, Wang L, Wang H, Luo X, Chen Q, Liu K, Shang Z
Journal of cellular physiology 2020 Sep;235(9):5995-6009

UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.

Sun J, Zhu Z, Li W, Shen M, Cao C, Sun Q, Guo Z, Liu L, Wu D
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5

Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2.

Zhan Y, Li R, Feng C, Li X, Huang S, Wang L, Liu Z, Jiang J, Han Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Jan;121:109602

Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.

Li C, Zheng H, Hou W, Bao H, Xiong J, Che W, Gu Y, Sun H, Liang P
Cell death & disease 2019 Sep 26;10(10):717

R-spondin1/Wnt-enhanced Ascl2 autoregulation controls the self-renewal of colorectal cancer progenitor cells.

Ye J, Liu S, Shang Y, Chen H, Wang R
Cell cycle (Georgetown, Tex.) 2018;17(8):1014-1025

Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128-1-3p in Hirschsprung's disease.

Su Y, Wen Z, Shen Q, Zhang H, Peng L, Chen G, Zhu Z, Du C, Xie H, Li H, Xia Y, Tang W
Cell cycle (Georgetown, Tex.) 2018;17(4):459-467

Downregulation of MiR-203a Disinhibits Bmi1 and Promotes Growth and Proliferation of Keratinocytes in Cholesteatoma.

Zang J, Hui L, Yang N, Yang B, Jiang X
International journal of medical sciences 2018;15(5):447-455

SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.

Xie SL, Fan S, Zhang SY, Chen WX, Li QX, Pan GK, Zhang HQ, Wang WW, Weng B, Zhang Z, Li JS, Lin ZY
International journal of cancer 2018 Mar 15;142(6):1252-1265

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Wang X, Cao W, Zhang J, Yan M, Xu Q, Wu X, Wan L, Zhang Z, Zhang C, Qin X, Xiao M, Ye D, Liu Y, Han Z, Wang S, Mao L, Wei W, Chen W
The EMBO journal 2017 May 2;36(9):1243-1260

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Wei X, Ye J, Shang Y, Chen H, Liu S, Liu L, Wang R
Oncotarget 2017 Dec 12;8(65):109301-109318

Preclinical activity of the novel B-cell-specific Moloney murine leukemia virus integration site 1 inhibitor PTC-209 in acute myeloid leukemia: Implications for leukemia therapy.

Nishida Y, Maeda A, Chachad D, Ishizawa J, Qiu YH, Kornblau SM, Kimura S, Andreeff M, Kojima K
Cancer science 2015 Dec;106(12):1705-13

Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma.

Wang HB, Liu GH, Zhang H, Xing S, Hu LJ, Zhao WF, Xie B, Li MZ, Zeng BH, Li Y, Zeng MS
The FEBS journal 2013 Jun;280(12):2929-44

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

Wu Z, Min L, Chen D, Hao D, Duan Y, Qiu G, Wang Y
PloS one 2011 Feb 2;6(2):e14648

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

Kim MO, Chawla P, Overland RP, Xia E, Sadri-Vakili G, Cha JH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 9;28(15):3947-57

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: U-937 cells were subjected to SDS PAGE followed by western blot with 10832-1-AP(BMI1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using 10832-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver cancer using 10832-1-AP(BMI1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue