Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C288481 - Provider product page
- Provider
- LSBio
- Product name
- Anti-BMI1 / PCGF4 Antibody (aa276-326) LS-C288481
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Horse, Porcine, Rabbit, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human BMI1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human BMI1 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-BMI1 ( Lot4 - left and Lot3 - right) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.