HPA030472

antibody from Atlas Antibodies

Targeting: BMI1 PCGF4, RNF51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA030472

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA030472, RRID:AB_10601090

Product name

Anti-BMI1

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RPTCKRMKISHQRDGLTNAGELESDSGSDKANSPA
GGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTS
NSPSGNHQSSFANRPRKSSVNGSSATSSG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1, using Anti-BMI1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HDLM-2.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.

Sample type

HUMAN