600-101-392

antibody from Rockland Immunochemicals, Inc.

Targeting: BMI1 PCGF4, RNF51

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

600-101-392

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-101-392, RRID:AB_2065430

Product name

Anti-BMI1 (GOAT) Antibody - 600-101-392

Antibody type

Polyclonal

Vial size

100 µl

Immunocytochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunofluorescence using Rockland's affinity purified goat anti Bmi1 shows nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4°C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).

Sample type

IF Microscopy