- AHZ0492 - Invitrogen Antibodies CDK5 antibody

Submitted references Cyfip1 Regulates SynGAP1 at Hippocampal Synapses.

Sahasrabudhe A, Begum F, Guevara CA, Morrison C, Hsiao K, Kezunovic N, Bozdagi-Gunal O, Benson DL
Frontiers in synaptic neuroscience 2020;12:581714

Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.

Vevea JD, Chapman ER
eLife 2020 Jun 9;9

Effect of Melatonin on Tau aggregation and Tau-mediated cell surface morphology.

Das R, Balmik AA, Chinnathambi S
International journal of biological macromolecules 2020 Jun 1;152:30-39

Inhibition of Cdk5 induces cell death of tumor-initiating cells.

Mandl MM, Zhang S, Ulrich M, Schmoeckel E, Mayr D, Vollmar AM, Liebl J
British journal of cancer 2017 Mar 28;116(7):912-922

Inhibition of endothelial Cdk5 reduces tumor growth by promoting non-productive angiogenesis.

Merk H, Zhang S, Lehr T, Müller C, Ulrich M, Bibb JA, Adams RH, Bracher F, Zahler S, Vollmar AM, Liebl J
Oncotarget 2016 Feb 2;7(5):6088-104

Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2.

Liebl J, Zhang S, Moser M, Agalarov Y, Demir CS, Hager B, Bibb JA, Adams RH, Kiefer F, Miura N, Petrova TV, Vollmar AM, Zahler S
Nature communications 2015 Jun 1;6:7274

Osteosarcoma cells induce endothelial cell proliferation during neo-angiogenesis.

de Nigris F, Mancini FP, Schiano C, Infante T, Zullo A, Minucci PB, Al-Omran M, Giordano A, Napoli C
Journal of cellular physiology 2013 Apr;228(4):846-52

Protein kinase Czeta regulates Cdk5/p25 signaling during myogenesis.

de Thonel A, Ferraris SE, Pallari HM, Imanishi SY, Kochin V, Hosokawa T, Hisanaga S, Sahlgren C, Eriksson JE
Molecular biology of the cell 2010 Apr 15;21(8):1423-34

Effect of aged garlic extract on APP processing and tau phosphorylation in Alzheimer's transgenic model Tg2576.

Chauhan NB
Journal of ethnopharmacology 2006 Dec 6;108(3):385-94

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CDK5 was performed by loading 30 ug of HEK293 control (Lane 1), HEK293 - CDK5 knockout (Lane 2) Edigene lysate. The blot was probed with Anti-CDK5 Monoclonal Antibody (Product # AHZ0492, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to CDK5.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) of SH-SY5Y (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), PC-3 (Lane 4), MDA-MB-231 (Lane 5), A549 (Lane 6), HeLa (Lane 7) and HT-29 (Lane 8). The blot was probed with Anti-CDK5 Monoclonal Antibody (DC34) (Product # AHZ0492, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 33 kDa band corresponding to CDK5 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CDK5 in MDA231, MAD109 and CCL-4 cells using a CDK5 monoclonal antibody (Product # AHZ0492).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CDK5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CDK5 Monoclonal Antibody (DC34) (Product # AHZ0492) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Cdk5 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk5 monoclonal antibody (Product # AHZ0492) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Cdk5 regulates NICD generation ( A ) Immunoblots from HUVECs transfected with nt (non-targeting) and Cdk5 siRNA and plated onto PBS- or DLL4-coated dishes probed with anti-NICD, anti-Cdk5, and anti-actin antibodies are shown. n = 3. The bar graph displays the quantification of the NICD immunoblot normed to the loading control (One Way ANOVA, Tukey, SEM, * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Cdk5 regulates Notch pathway dependent endothelial functions ( A ) Proliferation of HUVECs transfected with nt (non-targeting) or Cdk5 siRNA and plated onto PBS or Dll4 is shown. One-Way ANOVA, Tukey, SEM * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunoprecipitation of CDK5 in native human LS174T cells using a CDK5 monoclonal antibody (Product # AHZ0492).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 2 Reduced synaptic SynGAP and Cdk5 activity. Area CA1 in Wt and Cyfip1 +/- mice was imaged at high magnification in sections immunolabeled for SynGAP1 [green; (A) ] and Homer1b/c [red; (A) ], analyzed using ImageJ [overlay mask in panel (A) ]. Box plots show the mean area of overlap (B) and the integrated intensity of SynGAP puncta (C) in Wt and Cyfip1 +/- CA1. Data were compared using unpaired t -test, p = 0.0880 (B) and p = 0.0001 (C) . (D) Western blot for total and phosphorylated CaMKIIalpha (comparison values are in the text). (E) Western blots for Cdk5, its activator, p35, and a substrate, p-Synapsin-Ser 551 in Wt and Cyfip1 +/- mutant mouse hippocampi (comparison values are in the text).

AHZ0492

Antibody data