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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54486 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CDK5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.05 µl, 0.2 µl
- Storage
- Aliquot the CDK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A549, 2) K562, 3) HeLa and 4) mouse NIH 3T3 cell lysate with CDK5 antibody. Predicted molecular weight ~33 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human A549 cells with CDK5 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skeletal muscle tissue with CDK5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human K562 cells with CDK5 antibody; Blue=isotype control, Green= CDK5 antibody.
