TA505909

antibody from OriGene

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505909

Provider

OriGene

Proper citation

OriGene Cat#TA505909, RRID:AB_2623462

Product name

MAPK9 (JNK2) mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Antibody type

Monoclonal

Description

MAPK9 (JNK2) mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK9

Isotype

IgG

Antibody clone number

OTI2G3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAPK9 mouse monoclonal antibody (TA505909) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK9(RC207608).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909)

Validation comment

IHC