MA5-29409

antibody from Invitrogen Antibodies

Targeting: MAPK9 JNK2, p54a, PRKM9, SAPK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29409

Provider

Invitrogen Antibodies

Product name

JNK2 Recombinant Rabbit Monoclonal Antibody (011)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

11

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JNK2 in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a JNK2 Monoclonal Antibody (Product # MA5-29409) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:48 kDa. Observed band size:54 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 54 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human JNK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409, 1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human JNK2 in human kidney with JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409, 1:1,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human JNK2 with JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409) rabbit mnoclonal antibody (1:1,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

JNK2 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate, Lane C: 0.5 mg Jurkat Whole Cell Lysate, Lane D: 0.5 mg MCF-7 Whole Cell Lysate 0.5 µL with JNK2 Recombinant Rabbit Monoclonal Antibody (11) (Product # MA5-29409) and 15 µL of 50 % Protein G agarose. Primary antibody: JNK2 Recombinant Rabbit Monoclonal Antibody (11), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.