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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- V3390 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- N-Cadherin Antibody / CDH2
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 13A9
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the N-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1.
Selective mode of action of plumbagin through BRCA1 deficient breast cancer stem cells.
Central role for GSK3β in the pathogenesis of arrhythmogenic cardiomyopathy.
Characterization of matrix metalloproteinase-2 and -9, ADAM-10 and N-cadherin expression in human glioblastoma multiforme.
Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A.
Effects of human arylamine N-acetyltransferase I knockdown in triple-negative breast cancer cell lines.
ADAM-10 could mediate cleavage of N-cadherin promoting apoptosis in human atherosclerotic lesions leading to vulnerable plaque: a morphological and immunohistochemical study.
The cytoplasmic domain of N-cadherin modulates MMP‑9 induction in oral squamous carcinoma cells.
Mammary epithelial cell interactions with fibronectin stimulate epithelial-mesenchymal transition.
Male sex as a risk factor for the clinical course of skull base chordomas.
ΔNp63α activates CD82 metastasis suppressor to inhibit cancer cell invasion.
Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells.
N-cadherin has a protective role in stable human atherosclerotic plaques: a morphological and immunohistochemical study
Loss of focal adhesion kinase enhances endothelial barrier function and increases focal adhesions.
HYS-32, a novel analogue of combretastatin A-4, enhances connexin43 expression and gap junction intercellular communication in rat astrocytes.
Molecular prognostic prediction for locally advanced nasopharyngeal carcinoma by support vector machine integrated approach.
Lysophosphatidic acid modulates the association of PTP1B with N-cadherin/catenin complex in SKOV3 ovarian cancer cells.
Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model.
Epithelial-mesenchymal transition biomarkers and support vector machine guided model in preoperatively predicting regional lymph node metastasis for rectal cancer.
Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus.
Native type IV collagen induces an epithelial to mesenchymal transition-like process in mammary epithelial cells MCF10A.
Hyaluronan suppresses prostate tumor cell proliferation through diminished expression of N-cadherin and aberrant growth factor receptor signaling.
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Multiple lymphadenopathy as an initial sign of extramammary Paget disease.
Linoleic acid induces an EMT-like process in mammary epithelial cells MCF10A.
Liquid-diet with alcohol alters maternal, fetal and placental weights and the expression of molecules involved in integrin signaling in the fetal cerebral cortex.
The PX-RICS-14-3-3zeta/theta complex couples N-cadherin-beta-catenin with dynein-dynactin to mediate its export from the endoplasmic reticulum.
Arachidonic acid promotes epithelial-to-mesenchymal-like transition in mammary epithelial cells MCF10A.
Huang H, Svoboda RA, Lazenby AJ, Saowapa J, Chaika N, Ding K, Wheelock MJ, Johnson KR
The Journal of biological chemistry 2016 Oct 28;291(44):23208-23223
The Journal of biological chemistry 2016 Oct 28;291(44):23208-23223
Selective mode of action of plumbagin through BRCA1 deficient breast cancer stem cells.
Somasundaram V, Hemalatha SK, Pal K, Sinha S, Nair AS, Mukhopadhyay D, Srinivas P
BMC cancer 2016 May 26;16:336
BMC cancer 2016 May 26;16:336
Central role for GSK3β in the pathogenesis of arrhythmogenic cardiomyopathy.
Chelko SP, Asimaki A, Andersen P, Bedja D, Amat-Alarcon N, DeMazumder D, Jasti R, MacRae CA, Leber R, Kleber AG, Saffitz JE, Judge DP
JCI insight 2016 Apr 21;1(5)
JCI insight 2016 Apr 21;1(5)
Characterization of matrix metalloproteinase-2 and -9, ADAM-10 and N-cadherin expression in human glioblastoma multiforme.
Musumeci G, Magro G, Cardile V, Coco M, Marzagalli R, Castrogiovanni P, Imbesi R, Graziano AC, Barone F, Di Rosa M, Castorina S, Castorina A
Cell and tissue research 2015 Oct;362(1):45-60
Cell and tissue research 2015 Oct;362(1):45-60
Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A.
Galindo-Hernandez O, Gonzales-Vazquez C, Cortes-Reynosa P, Reyes-Uribe E, Chavez-Ocaña S, Reyes-Hernandez O, Sierra-Martinez M, Salazar EP
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Dec;36(12):9649-59
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Dec;36(12):9649-59
Effects of human arylamine N-acetyltransferase I knockdown in triple-negative breast cancer cell lines.
Tiang JM, Butcher NJ, Minchin RF
Cancer medicine 2015 Apr;4(4):565-74
Cancer medicine 2015 Apr;4(4):565-74
ADAM-10 could mediate cleavage of N-cadherin promoting apoptosis in human atherosclerotic lesions leading to vulnerable plaque: a morphological and immunohistochemical study.
Musumeci G, Coleman R, Imbesi R, Magro G, Parenti R, Szychlinska MA, Scuderi R, Cinà CS, Castorina S, Castrogiovanni P
Acta histochemica 2014 Sep;116(7):1148-58
Acta histochemica 2014 Sep;116(7):1148-58
The cytoplasmic domain of N-cadherin modulates MMP‑9 induction in oral squamous carcinoma cells.
Walker A, Frei R, Lawson KR
International journal of oncology 2014 Oct;45(4):1699-706
International journal of oncology 2014 Oct;45(4):1699-706
Mammary epithelial cell interactions with fibronectin stimulate epithelial-mesenchymal transition.
Park J, Schwarzbauer JE
Oncogene 2014 Mar 27;33(13):1649-57
Oncogene 2014 Mar 27;33(13):1649-57
Male sex as a risk factor for the clinical course of skull base chordomas.
Rachinger W, Eigenbrod S, Dützmann S, Simon M, Feigl GC, Kremenevskaja N, Kretzschmar H, Zausinger S, Kreth FW, Thon N, Tonn JC
Journal of neurosurgery 2014 Jun;120(6):1313-20
Journal of neurosurgery 2014 Jun;120(6):1313-20
ΔNp63α activates CD82 metastasis suppressor to inhibit cancer cell invasion.
Wu J, Liang S, Bergholz J, He H, Walsh EM, Zhang Y, Xiao ZX
Cell death & disease 2014 Jun 5;5:e1280
Cell death & disease 2014 Jun 5;5:e1280
Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells.
Galindo-Hernandez O, Serna-Marquez N, Castillo-Sanchez R, Salazar EP
Prostaglandins, leukotrienes, and essential fatty acids 2014 Dec;91(6):299-310
Prostaglandins, leukotrienes, and essential fatty acids 2014 Dec;91(6):299-310
N-cadherin has a protective role in stable human atherosclerotic plaques: a morphological and immunohistochemical study
Musumeci G, Imbesi R, Magro G, Parenti R, Szychlinska M, Scuderi R, Castorina S, Castrogiovanni P
Journal of Histology and Histopathology 2014 ;1(1):4
Journal of Histology and Histopathology 2014 ;1(1):4
Loss of focal adhesion kinase enhances endothelial barrier function and increases focal adhesions.
Arnold KM, Goeckeler ZM, Wysolmerski RB
Microcirculation (New York, N.Y. : 1994) 2013 Oct;20(7):637-49
Microcirculation (New York, N.Y. : 1994) 2013 Oct;20(7):637-49
HYS-32, a novel analogue of combretastatin A-4, enhances connexin43 expression and gap junction intercellular communication in rat astrocytes.
Lin PC, Shen CC, Liao CK, Jow GM, Chiu CT, Chung TH, Wu JC
Neurochemistry international 2013 May;62(6):881-92
Neurochemistry international 2013 May;62(6):881-92
Molecular prognostic prediction for locally advanced nasopharyngeal carcinoma by support vector machine integrated approach.
Wan XB, Zhao Y, Fan XJ, Cai HM, Zhang Y, Chen MY, Xu J, Wu XY, Li HB, Zeng YX, Hong MH, Liu Q
PloS one 2012;7(3):e31989
PloS one 2012;7(3):e31989
Lysophosphatidic acid modulates the association of PTP1B with N-cadherin/catenin complex in SKOV3 ovarian cancer cells.
Huang RY, Wen CC, Liao CK, Wang SH, Chou LY, Wu JC
Cell biology international 2012 Sep;36(9):833-41
Cell biology international 2012 Sep;36(9):833-41
Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model.
Chen MC, Chang WW, Kuan YD, Lin ST, Hsu HC, Lee CH
Innate immunity 2012 Oct;18(5):685-93
Innate immunity 2012 Oct;18(5):685-93
Epithelial-mesenchymal transition biomarkers and support vector machine guided model in preoperatively predicting regional lymph node metastasis for rectal cancer.
Fan XJ, Wan XB, Huang Y, Cai HM, Fu XH, Yang ZL, Chen DK, Song SX, Wu PH, Liu Q, Wang L, Wang JP
British journal of cancer 2012 May 22;106(11):1735-41
British journal of cancer 2012 May 22;106(11):1735-41
Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus.
Beaudoin GM 3rd, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jan 4;32(1):99-110
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Jan 4;32(1):99-110
Native type IV collagen induces an epithelial to mesenchymal transition-like process in mammary epithelial cells MCF10A.
Espinosa Neira R, Salazar EP
The international journal of biochemistry & cell biology 2012 Dec;44(12):2194-203
The international journal of biochemistry & cell biology 2012 Dec;44(12):2194-203
Hyaluronan suppresses prostate tumor cell proliferation through diminished expression of N-cadherin and aberrant growth factor receptor signaling.
Bharadwaj AG, Goodrich NP, McAtee CO, Haferbier K, Oakley GG, Wahl JK 3rd, Simpson MA
Experimental cell research 2011 May 1;317(8):1214-25
Experimental cell research 2011 May 1;317(8):1214-25
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Katafiasz D, Smith LM, Wahl JK 3rd
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Multiple lymphadenopathy as an initial sign of extramammary Paget disease.
Hirakawa S, Tanemura A, Mori H, Katayama I, Hashimoto K
The British journal of dermatology 2011 Jan;164(1):200-3
The British journal of dermatology 2011 Jan;164(1):200-3
Linoleic acid induces an EMT-like process in mammary epithelial cells MCF10A.
Espinosa-Neira R, Mejia-Rangel J, Cortes-Reynosa P, Salazar EP
The international journal of biochemistry & cell biology 2011 Dec;43(12):1782-91
The international journal of biochemistry & cell biology 2011 Dec;43(12):1782-91
Liquid-diet with alcohol alters maternal, fetal and placental weights and the expression of molecules involved in integrin signaling in the fetal cerebral cortex.
Rout UK, Dhossche JM
International journal of environmental research and public health 2010 Nov;7(11):4023-36
International journal of environmental research and public health 2010 Nov;7(11):4023-36
The PX-RICS-14-3-3zeta/theta complex couples N-cadherin-beta-catenin with dynein-dynactin to mediate its export from the endoplasmic reticulum.
Nakamura T, Hayashi T, Mimori-Kiyosue Y, Sakaue F, Matsuura K, Iemura S, Natsume T, Akiyama T
The Journal of biological chemistry 2010 May 21;285(21):16145-54
The Journal of biological chemistry 2010 May 21;285(21):16145-54
Arachidonic acid promotes epithelial-to-mesenchymal-like transition in mammary epithelial cells MCF10A.
Martinez-Orozco R, Navarro-Tito N, Soto-Guzman A, Castro-Sanchez L, Perez Salazar E
European journal of cell biology 2010 Jun;89(6):476-88
European journal of cell biology 2010 Jun;89(6):476-88
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human brain lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free N-Cadherin Antibody (clone 13A9). Confirmation of Integrity and Purity of the Antibody.
