Antibody data
- Antibody Data
- Antigen structure
- References [37]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [6]
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Validation data
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- Product number
- NBP1-48309 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-48309, RRID:AB_10011059
- Product name
- Mouse Monoclonal N-Cadherin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins.
The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology.
Lymph-circulating tumor cells show distinct properties to blood-circulating tumor cells and are efficient metastatic precursors.
Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression.
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.
N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration.
HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model.
Sonic hedgehog signalling regulates the self-renewal and proliferation of skin-derived precursor cells in mice.
Physical disruption of cell-cell contact induces VEGF expression in RPE cells.
The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy.
Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1.
The Enhanced metastatic potential of hepatocellular carcinoma (HCC) cells with sorafenib resistance.
N-cadherin switching occurs in high Gleason grade prostate cancer.
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
Structure and function of the N-cadherin/catenin complex in retinoblastoma.
Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells.
Expression of E-, P-, n-cadherins and catenins in human bladder carcinoma cell lines.
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression.
N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis?
N-cadherin redistribution during synaptogenesis in hippocampal neurons.
Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization.
Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization.
N-cadherin promotes adhesion between invasive breast cancer cells and the stroma.
Expression of E-cadherin and N-cadherin in surface epithelial-stromal tumors of the ovary distinguishes mucinous from serous and endometrioid tumors.
Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
Hlavac N, VandeVord PJ
Frontiers in neurology 2019;10:99
Frontiers in neurology 2019;10:99
The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology.
Chiba Y, He B, Yoshizaki K, Rhodes C, Ishijima M, Bleck CKE, Stempinski E, Chu EY, Nakamura T, Iwamoto T, de Vega S, Saito K, Fukumoto S, Yamada Y
The Journal of biological chemistry 2019 Mar 8;294(10):3406-3418
The Journal of biological chemistry 2019 Mar 8;294(10):3406-3418
Lymph-circulating tumor cells show distinct properties to blood-circulating tumor cells and are efficient metastatic precursors.
Mohammed SI, Torres-Luquis O, Walls E, Lloyd F
Molecular oncology 2019 Jun;13(6):1400-1418
Molecular oncology 2019 Jun;13(6):1400-1418
Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression.
Zhao K, Li X, Chen X, Zhu Q, Yin F, Ruan Q, Xia J, Niu Z
European journal of pharmacology 2019 Jul 5;854:298-306
European journal of pharmacology 2019 Jul 5;854:298-306
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.
Ren W, Hou J, Yang C, Wang H, Wu S, Wu Y, Zhao X, Lu C
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration.
Chen CY, Melo E, Jakob P, Friedlein A, Elsässer B, Goettig P, Kueppers V, Delobel F, Stucki C, Dunkley T, Fauser S, Schilling O, Iacone R
Matrix biology : journal of the International Society for Matrix Biology 2018 Sep;70:84-101
Matrix biology : journal of the International Society for Matrix Biology 2018 Sep;70:84-101
HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model.
Melo E, Oertle P, Trepp C, Meistermann H, Burgoyne T, Sborgi L, Cabrera AC, Chen CY, Hoflack JC, Kam-Thong T, Schmucki R, Badi L, Flint N, Ghiani ZE, Delobel F, Stucki C, Gromo G, Einhaus A, Hornsperger B, Golling S, Siebourg-Polster J, Gerber F, Bohrmann B, Futter C, Dunkley T, Hiller S, Schilling O, Enzmann V, Fauser S, Plodinec M, Iacone R
EBioMedicine 2018 Jan;27:258-274
EBioMedicine 2018 Jan;27:258-274
Sonic hedgehog signalling regulates the self-renewal and proliferation of skin-derived precursor cells in mice.
Park S, Kim H, Kim K, Roh S
Cell proliferation 2018 Dec;51(6):e12500
Cell proliferation 2018 Dec;51(6):e12500
Physical disruption of cell-cell contact induces VEGF expression in RPE cells.
Farjood F, Vargis E
Molecular vision 2017;23:431-446
Molecular vision 2017;23:431-446
The significance of intercalated discs in the pathogenesis of Friedreich cardiomyopathy.
Koeppen AH, Becker AB, Feustel PJ, Gelman BB, Mazurkiewicz JE
Journal of the neurological sciences 2016 Aug 15;367:171-6
Journal of the neurological sciences 2016 Aug 15;367:171-6
Development of patient-derived xenograft models from a spontaneously immortal low-grade meningioma cell line, KCI-MENG1.
Michelhaugh SK, Guastella AR, Varadarajan K, Klinger NV, Parajuli P, Ahmad A, Sethi S, Aboukameel A, Kiousis S, Zitron IM, Ebrahim SA, Polin LA, Sarkar FH, Bollig-Fischer A, Mittal S
Journal of translational medicine 2015 Jul 15;13:227
Journal of translational medicine 2015 Jul 15;13:227
The Enhanced metastatic potential of hepatocellular carcinoma (HCC) cells with sorafenib resistance.
Chow AK, Ng L, Lam CS, Wong SK, Wan TM, Cheng NS, Yau TC, Poon RT, Pang RW
PloS one 2013;8(11):e78675
PloS one 2013;8(11):e78675
N-cadherin switching occurs in high Gleason grade prostate cancer.
Jaggi M, Nazemi T, Abrahams NA, Baker JJ, Galich A, Smith LM, Balaji KC
The Prostate 2006 Feb 1;66(2):193-9
The Prostate 2006 Feb 1;66(2):193-9
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR
Oncogene 2006 Aug 3;25(33):4595-604
Oncogene 2006 Aug 3;25(33):4595-604
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76
The Journal of biological chemistry 2003 May 9;278(19):17269-76
Structure and function of the N-cadherin/catenin complex in retinoblastoma.
Van Aken EH, Papeleu P, De Potter P, Bruyneel E, Philippé J, Seregard S, Kvanta A, De Laey JJ, Mareel MM
Investigative ophthalmology & visual science 2002 Mar;43(3):595-602
Investigative ophthalmology & visual science 2002 Mar;43(3):595-602
Truncated DCC reduces N-cadherin/catenin expression and calcium-dependent cell adhesion in neuroblastoma cells.
Reyes-Múgica M, Meyerhardt JA, Rzasa J, Rimm DL, Johnson KR, Wheelock MJ, Reale MA
Laboratory investigation; a journal of technical methods and pathology 2001 Feb;81(2):201-10
Laboratory investigation; a journal of technical methods and pathology 2001 Feb;81(2):201-10
Expression of E-, P-, n-cadherins and catenins in human bladder carcinoma cell lines.
Mialhe A, Levacher G, Champelovier P, Martel V, Serres M, Knudsen K, Seigneurin D
The Journal of urology 2000 Sep;164(3 Pt 1):826-35
The Journal of urology 2000 Sep;164(3 Pt 1):826-35
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR
The Journal of cell biology 2000 Dec 11;151(6):1193-206
The Journal of cell biology 2000 Dec 11;151(6):1193-206
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR
The Journal of cell biology 2000 Dec 11;151(6):1193-206
The Journal of cell biology 2000 Dec 11;151(6):1193-206
N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression.
Nieman MT, Prudoff RS, Johnson KR, Wheelock MJ
The Journal of cell biology 1999 Nov 1;147(3):631-44
The Journal of cell biology 1999 Nov 1;147(3):631-44
N-cadherin-mediated human granulosa cell adhesion prevents apoptosis: a role in follicular atresia and luteolysis?
Makrigiannakis A, Coukos G, Christofidou-Solomidou M, Gour BJ, Radice GL, Blaschuk O, Coutifaris C
The American journal of pathology 1999 May;154(5):1391-406
The American journal of pathology 1999 May;154(5):1391-406
N-cadherin redistribution during synaptogenesis in hippocampal neurons.
Benson DL, Tanaka H
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 Sep 1;18(17):6892-904
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 Sep 1;18(17):6892-904
Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization.
Navarro P, Ruco L, Dejana E
The Journal of cell biology 1998 Mar 23;140(6):1475-84
The Journal of cell biology 1998 Mar 23;140(6):1475-84
Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization.
Navarro P, Ruco L, Dejana E
The Journal of cell biology 1998 Mar 23;140(6):1475-84
The Journal of cell biology 1998 Mar 23;140(6):1475-84
N-cadherin promotes adhesion between invasive breast cancer cells and the stroma.
Hazan RB, Kang L, Whooley BP, Borgen PI
Cell adhesion and communication 1997 Mar;4(6):399-411
Cell adhesion and communication 1997 Mar;4(6):399-411
Expression of E-cadherin and N-cadherin in surface epithelial-stromal tumors of the ovary distinguishes mucinous from serous and endometrioid tumors.
Peralta Soler A, Knudsen KA, Tecson-Miguel A, McBrearty FX, Han AC, Salazar H
Human pathology 1997 Jun;28(6):734-9
Human pathology 1997 Jun;28(6):734-9
Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
Han AC, Peralta-Soler A, Knudsen KA, Wheelock MJ, Johnson KR, Salazar H
Human pathology 1997 Jun;28(6):641-5
Human pathology 1997 Jun;28(6):641-5
Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues.
Han AC, Peralta-Soler A, Knudsen KA, Wheelock MJ, Johnson KR, Salazar H
Human pathology 1997 Jun;28(6):641-5
Human pathology 1997 Jun;28(6):641-5
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
The Journal of cell biology 1995 Jul;130(1):67-77
The Journal of cell biology 1995 Jul;130(1):67-77
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
The Journal of cell biology 1995 Jul;130(1):67-77
The Journal of cell biology 1995 Jul;130(1):67-77
The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
Peralta Soler A, Knudsen KA, Jaurand MC, Johnson KR, Wheelock MJ, Klein-Szanto AJ, Salazar H
Human pathology 1995 Dec;26(12):1363-9
Human pathology 1995 Dec;26(12):1363-9
The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas.
Peralta Soler A, Knudsen KA, Jaurand MC, Johnson KR, Wheelock MJ, Klein-Szanto AJ, Salazar H
Human pathology 1995 Dec;26(12):1363-9
Human pathology 1995 Dec;26(12):1363-9
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
The Journal of biological chemistry 1995 Aug 25;270(34):20201-6
The Journal of biological chemistry 1995 Aug 25;270(34):20201-6
Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
The Journal of biological chemistry 1995 Aug 25;270(34):20201-6
The Journal of biological chemistry 1995 Aug 25;270(34):20201-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with N-Cadherin Antibody (13A9)NBP1-48309R and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on A549 cells with N-Cadherin Antibody (13A9) NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on A431 cells with N-Cadherin Antibody (13A9) NBP1-48309G and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa Cells with N-Cadherin Antibody (13A9)NBP1-48309B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).