- MA5-15633 - Invitrogen Antibodies CDH2 antibody

Submitted references Re-expression of circ_0043610 contributes to trophoblast dysfunction through the miR-558/RYBP pathway in preeclampsia.

Shang J, Lin L, Huang X, Zhou L, Huang Q
Endocrine journal 2022 Dec 28;69(12):1373-1385

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

Shishkova DK, Velikanova EA, Bogdanov LA, Sinitsky MY, Kostyunin AE, Tsepokina AV, Gruzdeva OV, Mironov AV, Mukhamadiyarov RA, Glushkova TV, Krivkina EO, Matveeva VG, Hryachkova ON, Markova VE, Dyleva YA, Belik EV, Frolov AV, Shabaev AR, Efimova OS, Popova AN, Malysheva VY, Kolmykov RP, Sevostyanov OG, Russakov DM, Dolganyuk VF, Gutakovsky AK, Zhivodkov YA, Kozhukhov AS, Brusina EB, Ismagilov ZR, Barbarash OL, Yuzhalin AE, Kutikhin AG
International journal of molecular sciences 2021 Nov 18;22(22)

Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions.

Shishkova D, Markova V, Sinitsky M, Tsepokina A, Frolov A, Zagorodnikov N, Bogdanov L, Kutikhin A
International journal of molecular sciences 2020 Oct 28;21(21)

Calciprotein Particles Cause Endothelial Dysfunction under Flow.

Shishkova D, Markova V, Sinitsky M, Tsepokina A, Velikanova E, Bogdanov L, Glushkova T, Kutikhin A
International journal of molecular sciences 2020 Nov 20;21(22)

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Kutikhin AG, Tupikin AE, Matveeva VG, Shishkova DK, Antonova LV, Kabilov MR, Velikanova EA
Cells 2020 Apr 3;9(4)

MicroRNA‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells.

Alcantara KMM, Garcia RL
Oncology reports 2019 Apr;41(4):2103-2116

Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway.

Chen F, Zhuang M, Peng J, Wang X, Huang T, Li S, Lin M, Lin H, Xu Y, Li J, Chen Z, Huang Y
Molecular medicine reports 2014 Oct;10(4):1999-2003

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on tissue extract (30 µg lysate) of Mouse Brain (Lane 1). The blot was probed with Anti-N-cadherin Monoclonal Antibody (Product # MA5-15633, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 130 kDa band corresponding to N-cadherin was observed in the tissue tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of CD325/N Cadherin using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) in A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of A431 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of A431 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human placenta tissues using CD325/N Cadherin monoclonal antibody (Product # MA5-15633).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer (D), using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometric analysis of PC-2 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (green) and negative control (purple).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Systemic treatment with CPPs probably provokes EndoMT in rat endothelium. ( A ) Western blotting measurements of EndoMT transcription factors Snail and Slug and EndoMT marker N-cadherin as compared to the expression of vimentin, CD31 and VE-cadherin in HCAEC and HITAEC co-incubated with PBS, MPP, CPP-P or CPP-S in a flow system for 4 h. Blot scans (top) and band densitometry analysis (bottom). The results of the latter are represented by a heat map. Green, gray and red colours mean fold change =1.25, respectively, compared to PBS group; ( B ) gene expression analysis of SNAI1 , SNAI2 , TWIST1 , ZEB1 , CDH2 , ACTA2 , KDR , PECAM1 and CDH5 genes in HCAEC and HITAEC co-incubated with PBS, MPP, CPP-P or CPP-S in a flow system for 4 h. RT-qPCR measurements, the results are represented by a heat map. Green, gray and red colours mean fold change =2.00, respectively, compared to PBS group; ( C ) Western blotting measurements of EndoMT transcription factors Snail, Slug and Twist1 and alpha-smooth muscle actin as compared to the expression of Cd31, Gapdh and histone H3 in the endothelial lysate collected from the descending aorta and aortic arch of Wistar rats which received consecutive tail vein injections of CPP-P, CPP-S or 0.9% NaCl (10 daily injections). Blot scans (left) and band densitometry analysis (right). The results of the latter are represented by a heat map. Green, gray and red colours mean fold change

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 9 Proteomic profiling for ECFC, HCAEC and HUVEC. ( A ) Dot blot profiling for 55 angiogenesis-related proteins confirms high similarity between ECFC and mature vascular EC yet also indicates minor differences between these cell lines and generally validating RNA-seq results. Count to the right represents a quantitation of measured angiogenesis-related proteins in terms of their relative expression in HCAEC, HUVEC, and ECFC; ( B ) Western blotting for endothelial phenotype-related markers verifies endothelial identity of PBMC-derived ECFC and demonstrates an intermediate phenotype of PBMC-derived ECFC as compared to HCAEC and HUVEC in terms of HEY2, LYVE1, VEGFR3, Snail and Slug expression. beta-tubulin was probed as a loading control. Count to the bottom represents a quantitation of measured endothelial phenotype-related markers in terms of their relative expression in HCAEC, HUVEC, and ECFC.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Profiling of key endothelial molecules in human coronary artery endothelial cells (HCAECs) co-cultured with either human internal thoracic artery endothelial cells (HITAECs) (HIT) or human saphenous vein endothelial cells (HSaVECs) (HSVs) for 6, 24, or 48 h. ( A ) RT-qPCR profiling, HIT signifies the ratio of transcript levels (measured as DeltaCt) in HCAECs co-cultured with HITAECs to those in HCAECs co-cultured with HSaVECs. HIT (Ctrl) and HSV (Ctrl) represent the ratios of transcript levels (measured as DeltaCt) in HCAECs co-cultured with HITAECs or HSaVECs to those in HCAEC monoculture. Results are represented as the heat map; green, gray, and red colours indicate fold change =2.00, respectively; ( B ) Western blotting measurements. HCA-HIT represents HCAECs co-cultured with HITAECs, HCA-HSV signifies HCAECs co-cultured with HSaVECs. HCA and HSV signify HCAEC and HSaVEC monocultures, respectively; ( C ) Semi-quantitative analysis of Western blotting results by densitometry. HIT represents the ratio of band density in HCAECs co-cultured with HITAECs to that in HCAECs co-cultured with HSaVECs. HIT (Ctrl) and HSV (Ctrl) abbreviate the ratios of band density in HCAECs co-cultured with HITAECs or HSaVECs to that in HCAEC monoculture. HCA (Ctrl) abbreviates the ratio of band density in HCAEC monoculture to that in HSaVEC monoculture. Snail + Slug are shown at both 56 and 29 kDa values. Results are represented as green, gray, and red colours on t

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 CPPs induce endothelial activation and endothelial-to-mesenchymal transition. ( A ) HCAECs and HITAECs were cultured in the presence of PBS, MPPs, CPP-P, or CPP-S (100 muL of particles per well of a 6-well plate, OD 650 = 0.08-0.10, for CPP-P and CPP-S: equal to 50 mug calcium or 1.2 x 10 5 OsteoSense 680EX-positive PKH67-negative events) for 4 h, and total RNA was extracted with the following expression profiling for the indicated genes (n = 3 wells per group). Heat map shows differentially expressed genes (fold change > 2) between groups. ( B ) HCAECs and HITAECs were cultured in the presence of PBS, MPPs, CPP-P, or CPP-S (100 muL of particles per well of a 6-well plate, OD 650 = 0.08-0.10, for CPP-P and CPP-S: equal to 50 mug calcium or 1.2 x 10 5 OsteoSense 680EX-positive PKH67-negative events) for 4 h. Conditioned media were profiled for interleukin-6 and -8 using an enzyme-linked immunosorbent assay (n = 6 wells per group). Each dot represents one well of the culture plate. Whiskers indicate the range, box bounds indicate the 25th-75th percentiles, and centre lines indicate the median. p -values are provided above the boxes (statistically significant differences are marked by asterisks), Kruskal-Wallis test with post hoc false discovery rate correction by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli. ( C ) Conditioned media and cell lysate from HCAECs and HITAECs from the experiment in ( B ) were profiled using a cytokine array. N

MA5-15633

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