Explore
Validate
Learn
Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-65397 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65397, RRID:AB_962624
- Product name
- Rat Monoclonal TfR (Transferrin R) Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.125 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TfR (Transferrin R) Antibody (YTA74.4) [NB100-65397] - SWAP-70-deficient mice have lower erythropoietic reserves. Upper plots: Ter119(low)CD71(high) comprise proerythroblasts and Ter119+ comprise total erythroblasts. Percentages shown are of total BM and spleen cells. Lower plots: erythroblasts, subgated Ter119high. Percentages shown are of parent populations. (C) Automated-quantified (MACSQuant, Miltenyi) total numbers of Ter119+ cells in the BM (per 1 femur and 1 tibia) and in the spleen of wt and Swap-70(-/-) mice of different ages.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TfR (Transferrin R) Antibody (YTA74.4) [NB100-65397] - Alpha4 and beta1 integrin activity regulation is required for erythroblast developmental transition. (A) Representative FACS plots of bone marrow (BM) Ter119+ erythroblasts after treatment with anti-alpha4 blocking antibodies (Abs) or with isotype-matched Abs. Percentages shown are of parent populations. (B) and (C) Histograms showing the mean of two independent experiments for mice treated with anti-alpha4, anti-beta1, their combination or with iso-type-matched Abs.
