Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB2474-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TfR (Transferrin R) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TfR in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 29806
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands.
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Junctional expression of the prion protein PrPC by brain endothelial cells: a role in trans-endothelial migration of human monocytes.
Tremblay TL, Hill JJ
Scientific reports 2017 Apr 19;7:46574
Scientific reports 2017 Apr 19;7:46574
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud PO, Cazaubon S
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
Junctional expression of the prion protein PrPC by brain endothelial cells: a role in trans-endothelial migration of human monocytes.
Viegas P, Chaverot N, Enslen H, Perrière N, Couraud PO, Cazaubon S
Journal of cell science 2006 Nov 15;119(Pt 22):4634-43
Journal of cell science 2006 Nov 15;119(Pt 22):4634-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TfR (Transferrin R) in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human TfR (Transferrin R) Monoclonal Antibody (Catalog # MAB2474, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.