- 71-0300 - Invitrogen Antibodies GDI1 antibody

Submitted references Inhibiting glycolysis rescues memory impairment in an intellectual disability Gdi1-null mouse.

D'Adamo P, Horvat A, Gurgone A, Mignogna ML, Bianchi V, Masetti M, Ripamonti M, Taverna S, Velebit J, Malnar M, Muhič M, Fink K, Bachi A, Restuccia U, Belloli S, Moresco RM, Mercalli A, Piemonti L, Potokar M, Bobnar ST, Kreft M, Chowdhury HH, Stenovec M, Vardjan N, Zorec R
Metabolism: clinical and experimental 2021 Mar;116:154463

Inhibition of ER Stress by 2-Aminopurine Treatment Modulates Cardiomyopathy in a Murine Chronic Chagas Disease Model.

Ayyappan JP, Lizardo K, Wang S, Yurkow E, Nagajyothi JF
Biomolecules & therapeutics 2019 Jul 1;27(4):386-394

High fat diet aggravates cardiomyopathy in murine chronic Chagas disease.

Lizardo K, Ayyappan JP, Cui MH, Balasubramanya R, Jelicks LA, Nagajyothi JF
Microbes and infection 2019 Jan-Feb;21(1):63-71

Adipose Tissue Regulates Pulmonary Pathology during TB Infection.

Ayyappan JP, Ganapathi U, Lizardo K, Vinnard C, Subbian S, Perlin DS, Nagajyothi JF
mBio 2019 Apr 16;10(2)

Effect of Mycobacterium tuberculosis infection on adipocyte physiology.

Ayyappan JP, Vinnard C, Subbian S, Nagajyothi JF
Microbes and infection 2018 Feb;20(2):81-88

Diet regulates liver autophagy differentially in murine acute Trypanosoma cruzi infection.

Lizardo K, Almonte V, Law C, Aiyyappan JP, Cui MH, Nagajyothi JF
Parasitology research 2017 Feb;116(2):711-723

Antagonistic effect of atorvastatin on high fat diet induced survival during acute Chagas disease.

Zhao D, Lizardo K, Cui MH, Ambadipudi K, Lora J, Jelicks LA, Nagajyothi JF
Microbes and infection 2016 Nov;18(11):675-686

The transient expression of Klf4 and Klf5 during adipogenesis depends on GSK3β activity.

Cervantes-Camacho C, Beltrán-Langarica A, Ochoa-Uribe AK, Marsch-Moreno M, Ayala-Sumuano JT, Velez-delValle C, Kuri-Harcuch W
Adipocyte 2015 Oct-Dec;4(4):248-55

High fat diet modulates Trypanosoma cruzi infection associated myocarditis.

Nagajyothi F, Weiss LM, Zhao D, Koba W, Jelicks LA, Cui MH, Factor SM, Scherer PE, Tanowitz HB
PLoS neglected tropical diseases 2014 Oct;8(10):e3118

EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.

Imai A, Yoshie S, Ishibashi K, Haga-Tsujimura M, Nashida T, Shimomura H, Fukuda M
The Journal of biological chemistry 2011 Sep 30;286(39):33854-62

Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells.

Kuliawat R, Kalinina E, Bock J, Fricker L, McGraw TE, Kim SR, Zhong J, Scheller R, Arvan P
Molecular biology of the cell 2004 Apr;15(4):1690-701

Deletion of the mental retardation gene Gdi1 impairs associative memory and alters social behavior in mice.

D'Adamo P, Welzl H, Papadimitriou S, Raffaele di Barletta M, Tiveron C, Tatangelo L, Pozzi L, Chapman PF, Knevett SG, Ramsay MF, Valtorta F, Leoni C, Menegon A, Wolfer DP, Lipp HP, Toniolo D
Human molecular genetics 2002 Oct 1;11(21):2567-80

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

Greenfield JP, Leung LW, Cai D, Kaasik K, Gross RS, Rodriguez-Boulan E, Greengard P, Xu H
The Journal of biological chemistry 2002 Apr 5;277(14):12128-36

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HEK293 cell lysatess using Rb anti-GDI (Product # 71-0300).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of IMR32 (Lane 1), T98G (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HEK-293 (Lane 5).The blots were probed with GDI1 Rabbit polyclonal Antibody (Product # 71-0300, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~55 kDa band corresponding to GDI1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE&reg ; 4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Immunoblot analysis of the hearts confirmed T. cruzi infection induced systemic alterations in lipid/cholesterol homeostasis and macrophage infiltration. Protein levels of LDLr and oxidized LDLr (LOX1), LPL, Abca1 and HMGCR were altered in the hearts during T. cruzi infection. Macrophage infiltration marker (F4/80) was significantly increased in the infected RD fed mice compared to infected HFD fed mice. Fold change in the protein levels were normalized to GDI expression and represented as bar graph (n = 5, p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Immunoblot analysis of the WAT confirmed T. cruzi infection induced systemic alterations in lipid/cholesterol homeostasis. WAT from both the RD and HFD fed infected mice showed upregulated LDLr, Abca1 and LPL activity. Unlike hearts from the infected mice WAT showed no multimer adiponectin and also significantly reduced adiponectin monomers. Fold change in the protein levels were normalized to GDI expression and represented as bar graph (n = 5, p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 Immunoblot analysis of the hearts demonstrated altered adiponectin levels during acute T. cruzi infection. Immunoblot analysis demonstrated increased expression of adiponectin (multimer) in the hearts of infected mice compared to uninfected mice both in RD and HFD fed mice. Adiponectin monomer levels were significantly decreased in the hearts of infected mice. However, immunoblot analysis demonstrated significantly different levels of adiponectin levels between HFD and RD fed mice both at uninfected and infected conditions. Fold change in the protein levels were normalized to GDI expression and represented as bar graph (n = 5, p

71-0300

Antibody data