21146-1-AP
antibody from Invitrogen Antibodies
Targeting: LARS1
FLJ10595, FLJ21788, HSPC192, LARS, LEUS, RNTLS
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
- Other assay [1]
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Validation data
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- Product number
- 21146-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EFQAAKDKY RELAVEGMHR ELVFRFIEVQ TLLLAPFCPH LCEHIWTLLG KPDSIMNASW PVAGPVNEVL IHSSQYLMEV THDLRLRLKN YMMPAKGKKT DKQPLQKPSH CTIYVAKNYP PWQHTTLSVL RKHFEANNGK LPDNKVIASE LGSMPELKKY MKKVMPFVAM IKENLEKMGP RILDLQLEFD EKAVLMENIV YLTNSLELEH IEVKFASEAE DKIREDCCPG KPLNVFRIEP GVSVSLVNPQ PSNGHFSTKI EIRQGDNCDS IIRRLMKMNR GIKDLSKVKL MRFDDPLLGP RRVPVLGKEY TEKTPISEHA VFNVDLMSKK IHLTENGIRV DIGDTIIYLV H (827-1176 aa encoded by BC151214)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LARS antibody (Product # 21146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using LARS antibody 21146-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 21146-1-AP (LARS antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 21146-1-AP ( LARS Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 21146-1-AP ( LARS Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LARS (IP:21146-1-AP, 4ug; Detection:21146-1-AP 1:500) with Jurkat cells lysate 2400ug.