Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 21749-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21749-1-AP, RRID:AB_10858789
- Product name
- HIGD1A antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated HIGD1A antibody (Cat. #21749-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references HIG1 domain family member 1A disrupts proliferation, migration, and invasion of colon adenocarcinoma cells.
HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
Higd1a improves respiratory function in the models of mitochondrial disorder.
Higd1a is a positive regulator of cytochrome c oxidase.
Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Xu Z, Sun J, Mao Y, Chen Y, Zhang T, Qin Y, Hua D
Bioengineered 2021 Dec;12(2):10501-10511
Bioengineered 2021 Dec;12(2):10501-10511
HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
Hock DH, Reljic B, Ang CS, Muellner-Wong L, Mountford HS, Compton AG, Ryan MT, Thorburn DR, Stroud DA
Molecular & cellular proteomics : MCP 2020 Jul;19(7):1145-1160
Molecular & cellular proteomics : MCP 2020 Jul;19(7):1145-1160
Higd1a improves respiratory function in the models of mitochondrial disorder.
Nagao T, Shintani Y, Hayashi T, Kioka H, Kato H, Nishida Y, Yamazaki S, Tsukamoto O, Yashirogi S, Yazawa I, Asano Y, Shinzawa-Itoh K, Imamura H, Suzuki T, Suzuki T, Goto YI, Takashima S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):1859-1871
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):1859-1871
Higd1a is a positive regulator of cytochrome c oxidase.
Hayashi T, Asano Y, Shintani Y, Aoyama H, Kioka H, Tsukamoto O, Hikita M, Shinzawa-Itoh K, Takafuji K, Higo S, Kato H, Yamazaki S, Matsuoka K, Nakano A, Asanuma H, Asakura M, Minamino T, Goto Y, Ogura T, Kitakaze M, Komuro I, Sakata Y, Tsukihara T, Yoshikawa S, Takashima S
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 3;112(5):1553-8
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 3;112(5):1553-8
Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.
Ameri K, Rajah AM, Nguyen V, Sanders TA, Jahangiri A, Delay M, Donne M, Choi HJ, Tormos KV, Yeghiazarians Y, Jeffrey SS, Rinaudo PF, Rowitch DH, Aghi M, Maltepe E
PloS one 2013;8(4):e62758
PloS one 2013;8(4):e62758
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP(HIGD1A antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using HIGD1A antibody 21749-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 21749-1-AP(HIGD1A antibody) at dilution of 1:200 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 21749-1-AP(HIGD1A antibody) at dilution of 1:200 (under 40x lens)
- Sample type
- tissue